水稻选择导入系氮高效材料鉴定与qtl定位分析-identification of nitrogen efficient materials and qtl mapping analysis for rice selective introduction lines.docxVIP

在无氮水培条件下同样被筛选到是氮高效株系。5.筛出的优良材料与定位到的位点的分析与验证表明，群体蜀恢527/PU28中，标记RM402、RM289、RM219和RM19这4个连锁位点存在于氮高效株系中，其有利等位基因来自于供体亲本PU28，说明这些导入的片段可以用于轮回亲本的改良；明恢86/PU28群体中，标记RM108、RM252、RM32、RM508、RM5463、RM5699、RM6959、RM85和RM5349这9个连锁位点存在于氮高效株系中，其有利等位基因来自于供体亲本PU28；群体蜀恢527/爷驼崽中，标记RM160、RM287、RM3688和RM481这4个连锁位点存在于氮高效株系中，其有利等位基因来自于供体亲本爷驼崽；群体明恢86/爷驼崽，标记RM200、RM287、RM3307、RM349、RM448和RM511这6个连锁位点存在于氮高效株系中，其有利等位基因来自于供体亲本爷驼崽。这些重要位点在大部分优良株系中稳定表达，对于轮回亲本蜀恢527和明恢86的改良具有重要的指导意义。关键词：水稻；氮高效；水培；选择导入系；QTLIIAbstractNitrogenapplicationhasbeenoneoftheimportantapproachestoimprovericeyieldperunitarea.However,withtheincreasingofnitrogenfertilizing,nitrogenuseefficiencydecreased.Therefore,discoveringlownitrogentolerantricegene/QTL,developingnitrogenefficientvarieties,exploringandutilizethepotentialofriceisanurgentneedtoresolveproblemsinriceproduction.Inthisstudy,Shuhui527andMinghui86,thetwowidelyusedeliteindicarestorersinChina,wereusedasrecurrentparents,twoindicavarietiesYetuozaiandPU28asdonorfordevelopingfourBC2F5backcrossingintrogressionline(IL)populations.66,64,60and65SSRmarkerswereevenlydistributedon12chromosomesforgenotypeanalysis.Headingdate,plantheight,paniclelength,paniclenumberperplant,filled-grainnumberperpanicle,spikeletnumberperpanicle,spikeletfertility,thousandgrainweight,biomassoffourpopulationswereevaluatedunderthefieldduringthewholegrowthperiodofandchlorophyllcontent,rootlength,shoot,totaldryweightandrootshootratio(dryweight)andotherfivetraitswereanalyzedusinghydroponicculturemethodatseedlingswerecombiningwithQTLanalysis.Themainresultsareasfollows:Thephenotypicperformancesshowedthatlownitrogenstresshadlargeimpactonplantheight,paniclenumberperplant,spikeletfertilityandbiomassoffourpopulations,resultinginthereductionofgrainyieldperplantinfieldenvironment.Withthecontentof(NH4)2SO4decreasedinhydroponicculture,theparentandpopulationsroughlydisplayedthistrends,thatwaschlorophyllcontentdecreasedgradually,rootlengthincreased,seedlingheightdecreased,weightreducedandroot/shootratioincreased.Correlat