褪黑素对宫内感染胎鼠脑组织损伤的保护作用和mmp-9timp-1表达的影响-protective effect of melatonin on brain injury and mmp - 9 timp - 1 expression in intrauterine fetal rats.docxVIP

褪黑素对宫内感染胎鼠脑组织损伤的保护作用和 MMP-9/TIMP-1 表达的影响 英文摘要 英文摘要 褪黑素对宫内感染胎鼠脑组织损伤的保护作用和 MMP-9/TIMP-1 表达的影响  PAGE 3 IV The Protective effects of melatonin on oxidative damage and the expression of MMP-9/TIMP-1 of fetal brain in intrauterine infection model of rat Abstract Objective: To establish the intrauterine infection model of rat through intraperitoneal injection with bacterial lipopolysaccharide to pregnant rats, investigate the effects and mechanisms of melatonin (MT) on ability of anti-injury of fetal brain induced by intrauterine infection of the fetal rat brain damage caused by bacterial lipopolysaccharide, for the further study of the clinical application of MT in a obstetric experimental basis. Methods: The 19th days of pregnant Sprague-Dawley rats were randomly divided into three groups: the blank control group, the intrauterine infection group(LPS group) and the melatonin group (MT group). To establish the models, fetal rat brain damage made by lipopolysaccharide (LPS)-induced intrauterine infection. In LPS group, the pregnant rats received an intraperitoneal injection of LPS (500 μg/kg). In MT group, the rats were treated with LPS 500μg/kg and MT 10 mg/kg simultaneously. Then, according the different observation time after injection, the pregnant rats were divided into six time points (n=4) respectively: 1h, 6h, 12h, 24h, 48h, 72h. Pregnant rats were sacrificed at the appropriate point quickly by caesarean section on the ice box, and remove the fetal rat brain and placental tissues. Detected the activities of superoxide dismutase (SOD) in fetal brain homogenates and the levels of MMP-9mRNA and TIMP-1mRNA expression in fetal rat brain tissue by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). In addition, the ratio of MMP-9/TIMP-1mRNA were calculated. Meanwhile, the pathological changes of placenta and brain tissues were observed under light microscope by HE staining.  Results: The intrauterine infection models of rat were constructed successfull