维a酸注射液对裸鼠人肝癌细胞smmc-7721移植瘤的抑制作用-inhibitory effect of retinoic acid injection on smmc - 7721 transplanted tumor in nude mice.docxVIP

PAGE PAGE 10 Inhibitory effect of tretinoin injection on hepatocellular carcinoma cells SMMC-7721 xenograft in nude mice Abstract: Objective: Tretinoin is one of the mature retinoid acid drugs for tumor chemical treatment and prevention in clinic at the present time. It can be used for tumor chemical prevention and treatment. Based on the previous studies, to study the effect of tretinoin injection on the growth of hepatocellular carcinoma cells SMMC-7721 in vitro and nude mice tumor xenograft in vivo, to detect tumor tissue cell apoptosis and caspase-3 expression, to aims to clarify tretinoin injection action and possible mechanism of anti-liver tumor, and to provide a scientific experiment basis for its clinical application on the treatment of liver cancer. Methods: (1) Take SMMC-7721 cells in the growing period of logarithm to be intervented by different concentration tretinoin injection (final concentration are 100 μg/mL, 50 μg/mL, 25 μg/mL, 12.5 μg/mL, 6.25 μg/mL and 3.125 μg/mL) for 48 hours. The negative control was culture medium for equal volume. The positive control was anti-cancer drug cisplatin (DDP), then its final concentration was respective 10 μg/mL, 1 μg/mL and 0.1 μg/mL. The CCK-8 Kit (Cell Counting Kit-8) was used to test the influence for human liver cells SMMC-7721 proliferation; (2) To establish the transplant tumor model in nude mice for human liver cells SMMC-7721: After 15 days, the nude mice, with developed tumors≥50 mm3 were chosen and the nude mice were removed with transplant tumor no growth or transplant tumor slow growth or transplant tumor fast growth. Based on the experiment scheme, nude mice were randomly divided into 5 groups: negative control group(saline), positive control group (cisplatin, 1mg/kg weight) and low-dosage, medium-dosage and high-dosage for tretinoin injection(7.5 mg/kg weight, 15 mg/kg weight and 30 mg/kg weight), by intraperitoneal injection. For a total of four weeks to medicine in five days given drug with