PM2.5暴露对鸦胆子油乳治疗荷瘤肺癌小鼠腹腔巨噬细胞作用.docVIP

PM2.5暴露对鸦胆子油乳治疗荷瘤肺癌小鼠腹腔巨噬细胞作用 　　[摘要] 目的 探讨PM2.5暴露对鸦胆子油乳治疗荷瘤肺癌小鼠腹腔巨噬细胞的活性调节作用。 方法 建立肺癌荷瘤A549细胞小鼠模型32只，随机分为4组：治疗组1（鸦胆子油乳治疗组）和对照组1，治疗组2（PM2.5暴露联合鸦胆子油乳治疗组）和对照组2，每组8只。收集腹腔巨噬细胞，检测520 nm吸光度值。 结果 腹腔巨噬细胞吞噬能力显示，治疗组1高于对照组1，差异有统计学意义（P0.05）；治疗组2低于对照组2及治疗组1高于治疗组2，差异有统计学意义（P0.01）。 结论 PM2.5暴露对鸦胆子油乳治疗荷瘤肺癌小鼠腹腔巨噬细胞的活性有抑制作用。 　　[关键词] 空气细颗粒物；鸦胆子油乳；荷瘤小鼠；腹腔巨噬细胞 　　[中图分类号] R332 [文献标识码] A [文章编号] 1674-4721（2016）02（c）-0015-04 　　[Abstract] Objective To explore active regulating effect of PM2.5 exposure for peritoneal macrophages of tumor-bearing mice with lung cancer treated by bruceolic oil emulsion. Methods 32 mouse models in tumor-bearing A549 cell in lung cancer were randomly divided into 4 groups：treatment group 1 （bruceolic oil emulsion treatment group） and control group 1，treatment group 2 （PM2.5 exposure combined with bruceolic oil emulsion treatment group） and control group 2.Each group had eight mice.Peritoneal macrophages from these mice were collected and 520 nm absorbance degree value was detected. Results phagocytic ability of peritoneal macrophage indicated that treatment group 1 was higher than that of control group 1，and there was a statistical difference （P0.05）.The phagocytic ability in the treatment group 2 was lower than that of control group 2，and the phagocytic ability in the treatment group 1 was higher than that of treatment group 2， and there was a statistical difference （P0.01）. Conclusion PM2.5 exposure has inhibiting effect for activity of peritoneal macrophages in tumor-bearing mice with lung cancer treated by bruceolic oil emulsion. 　　[Key words] Fine particulate matter in air；Bruceolic oil emulsion；Tumor-bearing mouse；Peritoneal macrophages 　　空气污染物由多种资源消耗的途径产生，并可以损伤人体的健康和破坏自然环境。空气污染物由室内和室外污染物组成，包括基本颗粒（灰尘和碳颗粒），气体成分（一氧化碳、氮气、硫氧化物和臭氧），有机复合物，部分农药（二 [恶]辛类）和某些生物制品材料，当这些污染物进入呼吸系统时，就可能有潜在的毒性作用[1]。对人类的健康来说，颗粒混合物的成分是最主要的危险因素。相关的文献报道了颗粒物组分与日常死亡率和入院率的相关性[2-3]。针对空气颗粒物的研究不仅可以为某些疾病的预防工作奠定基础，也可以更好地阐述受到空气污染后相关疾病的致病机制，通过发现新的细胞反应，提高对细胞途径的理解，最终发展成新的有效的预防和治疗疾病的手段。 　　鸦胆子油乳，是由中药鸦