Survivin和Livin在人骨肉瘤组织中表达及临床意义.docVIP

Survivin和Livin在人骨肉瘤组织中表达及临床意义 　　[摘要] 目的 研究Survivin和Livin在骨肉瘤中的表达及其临床意义。方法 对38例骨肉瘤及20例骨软骨瘤患者的临床病理资料进行回顾性分析,应用免疫组织化学染色结果进行统计学分析。结果 Survivin和Livin在38例标本中阳性率分别为44.7%(17/38)和55.3%(21/38),Survivin和Livin的表达与患者年龄、性别、骨肉瘤亚型无明显关系,但与Enneking外科分期有关,Ⅰ～ⅡA期与ⅡB～Ⅲ期之间肿瘤表达率差异明显(P0.05)。结论 Survivin和Livin在骨肉瘤组织中有较高比例的表达,可作为预测骨肉瘤预后的重要指标。 　　[关键词] 骨肉瘤;Survivin;Livin 　　[中图分类号] R738[中图分类号] A[文章编号] 1673-9701(2009)24-26-02 　　 　　Expression of Survivin and Livin in Human Osteosarcoma and Its Significance 　　YANG BaojunWANG Guizhi 　　Medical Group General Hospital of Pingdingshan Coal Leveling Industry,Henan 467000,China 　　 　　[Abstract] Objective To investigate the expression of survivin and livin in human osteosarcoma. Methods The clinical pathological data of 38 cases of osteosarcoma and 20 cases of osteochondroma were studied retrospectively and the statistical analysis was made of the results obtained by use of immunohistochemistry. Results The expression rates of survivin and livin in osteosarcoma was 44.7%(17/38) and 55.3%(21/38),respectively. The expression of survivin and livin was correlated with Enneking surgical staging. Its expression rate in Enneking surgical staging Ⅰ-ⅡA was significiantly lower than that in staging ⅡB-Ⅲ(P0.05). The expression of survivin and livin was not correlated with age and sex and pathological subtypes of osteosarcoma. Conclusion A high expression of survivin and livin is found in the osteosarcoma tissue,which can be used as an important index for predicting prognostic condition of human osteosarcoma. 　　[Key Words] Osteosarcoma;Survivin;Livin 　　 　　随着肿瘤分子遗传学与分子生物学的发展,凋亡作为一种程序性细胞死亡,广泛参与细胞的各种生理、病理过程,不仅对细胞发生、分化和维持机体稳态都有很重要的作用,而且还可以通过清除变异的细胞和改变基因表达阻止细胞发生转化。因此,如果凋亡被抑制,基因发生突变的细胞就会得以继续生存,从而通过突变导致肿瘤的发生和发展。本文采用免疫组化方法研究凋亡抑制蛋白Survivin和Livin在人骨肉瘤组织中的表达及其临床意义。 　　 　　1材料与方法 　　 　　1.1材料 　　收集河南省平顶山市平煤医疗集团总医院病理科1998～2007年诊断骨肉瘤病例38例,术前均未进行放疗和化疗,其中男性23例,女性15例,年龄7～43岁,平均年龄21岁,所有患者均手术治疗,诊断明确,另选取20例骨软骨瘤标本作为对照组。兔抗人Survivin多克隆抗体购于Labvision生物公司(Rb-6),兔抗人Livin多克隆抗体购于武汉博士德生物