Nogo受体拮抗剂对神经生长因子表达影响.docVIP

Nogo受体拮抗剂对神经生长因子表达影响 　　[摘要] 目的 探讨Nogo受体拮抗剂NEP1-40对大鼠脊髓损伤后，对神经生长因子（NGF）蛋白表达的影响。 方法 取雌性SD大鼠，随机分成对照组、生理盐水治疗组和NEP1-40治疗组，每组10只。建立T10节段的大鼠脊髓半切损伤模型，分别注射生理盐水和NEP1-40；于术后不同时间点对各组大鼠行后肢运动功能BBB评分；免疫组织化学染色检测神经生长因子（NGF）的表达。 结果 术后3周时，NEP1-40治疗组的BBB评分高于生理盐水治疗组（P0.05）；术后4周时，NEP1-40治疗组的BBB评分高于生理盐水治疗组（P0.01）。与生理盐水治疗组相比，NEP1-40治疗组在治疗2周后的神经生长因子（NGF）蛋白表达水平较高，差异有统计学意义（P0.05）。 结论 脊髓损伤后，NEP1-40可以增加神经生长因子（NGF）蛋白的表达，促进脊髓损伤后大鼠神经的再生。 　　[关键词] 脊髓损伤；Nogo受体拮抗剂；神经生长因子；再生 　　[中图分类号] R722.1 [文献标识码] A [文章编号] 1673-9701（2015）32-0027-03 　　[Abstract] Objective To discuss the effect of NEP1-40 on the expression of NGF in rats with spinal cord injury （SCI）. Methods 30 healthy adult Sprague-Dawley rats were randomly divided into model control normal group（n=10）， saline treatment group（n=10） and NEP1-40 treatment group （n=10）. Establish the model of acute spinal cord injury in T10. Injection of saline and NEP1-40 respectively. At different time points after operation， 5 rats in each group were assessed with Basso-Beattie-Bresnahan（BBB） scale， and the expression of Nerve Growth Factor of the spinal cord were detected with immunohistochemistry. Results The scores of BBB were better in NEP1-40 treatment group than in normal saline treatment group 3 weeks after SCI（P0.05）， The scores of BBB were better in NEP1-40 treatment group than in normal saline treatment group 4 weeks after SCI （P0.01）. Compared with normal saline treatment group， the expression of NGF increased 2 weeks after SCI in NEP1-40 treatment group（P0.05）. Conclusion NEP1-40 may promote the expression of NGF in spinal cord after SCI in order to improve neural regeneration in rats. 　　[Key words] Spinal cord injury； Nogo receptor antagonist； Nerve growth factor； Regeneration 　　脊髓损伤（spinal cord injury，SCI）是一种致残与致死率很高的疾病，其治疗一直是神经损伤修复领域的难题[1]。Nogo蛋白是目前所知有效的神经轴突生长的抑制因子[2]。Nogo受体拮抗剂（Nogo extra cellular peptide，residues1-40，NEP1-40）是Nogo受体（Nogo receptor，NgR）竞争性拮抗剂，能拮抗中枢神经抑制因子与Nogo受体的结合，间接促进新生轴突的生长，是目前国内外研究的热点[3]。本研究主要观察大鼠急性脊髓损伤后NEP1-40对运动功能恢复的影响及对神经生长