MTT法在细菌活性检测及药敏试验中应用.docVIP

MTT法在细菌活性检测及药敏试验中应用 　　MTT法在细菌活性检测及药敏试验中的应用 　　蔡燕 彭乙华 黄义山 杨明辉 罗书久 谢文光? 　　 　　基金项目：国家自然科学基金资助（项目批准号? 　　作者单位：637000 四川川北医学院附属医院? 　　通讯作者：谢文光 　　【摘要】 目的 探讨四甲基偶氮唑盐（MTT）法在细菌活性检测和药敏试验中的应用价值。方法 分别采用MTT法和微量肉汤稀释法检测鲍曼不动杆菌活性以及鲍曼不动杆菌对丁胺卡拉霉素和头孢他啶的敏感性，对两种方法的操作过程和结果进行比较。用统计软件SPSS 16.0对检测结果进行分析。结果 随着细菌浓度增加，微量肉汤稀释法和MTT法检测的活菌或死菌的吸光度值均增加，但用微量肉汤稀释法检测的同一浓度的活菌和死菌的吸光度值之间差别不大，t检验结果显示差异无统计学意义（P0.05）；而与用MTT法检测的同一浓度死菌吸光度值相比，MTT法检测的活菌吸光度值明显增加，t检验结果显示差异均有统计学意义（P0.05）；用微量肉汤稀释法和MTT法检测的鲍曼不动杆菌对丁胺卡拉霉素和头孢他啶的敏感性均与临床药敏结果一致。结论 同微量肉汤稀释法一样，MTT法可以用于检测细菌活性和药敏试验。? 　　 　　【关键词】 MTT法； 药敏试验； 微量肉汤稀释法； 鲍曼不动杆菌? 　　The application of MTT assay in detecting of bacteria’s activity and susceptibility test CAI Yan，PENG Yi-hua，HUANG Yi-shan，YANG Ming-hui，LUO Shu-jiu，XIE Weng-guang. Affiliated Hospital of North Sichuan Medical College，Nanchong 637000，China? 　　【Abstract】 Objective To research the applying value of MTT assay （Tetrazolium salt）in detecting the bacteria’s activity and drug sensitivity.Methods Using the MTT assay and broth microdilution methods to detect the Acinetobacter baumanii’s activity and the drug sensitivity of Acinetobacter baumanii to Amikaxinand Ceftazidime.The statistics software spss16.0 was applied to analyze the results.The periods and results of two methods were compared.Results With the concentration of bacteria increasing the optical density of every group detected by the MTT assay or broth microdilution also improved；But there were no difference between the optical density of dead bacteria and living bacteria in the same concentration detected by the broth microdilution method（P0.05）；When compared with the same concentration of the dead bacteria’s optical density， the optical density of the living bacteria detected by MTT assay increased significantly（P0.05）；The results of the drug susceptibility of Amikaxin and Ceftazidime detected by the MTT assay and the broth microdilution were consistent with the clinical consequence.Conclusion The MTT assay has the same function with the