HPV L1壳蛋白表达与宫颈病变严重程度关系研究.docVIP

HPV L1壳蛋白表达与宫颈病变严重程度关系研究 　　【摘要】目的：采用免疫组织化学法检测HPV L1壳蛋白的表达，并进一步分析研究其与宫颈病变的严重程度的关系。方法：选取我院妇科就诊患者的宫颈脱落细胞标本529例，所有患者进行薄层液基细胞学检查，同时行宫颈活检并采用免疫组织化学法检测HPV L1壳蛋白的表达，并且分析其与不同程度宫颈病变的相关性。结果：在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈鳞癌中，HPV L1壳蛋白的阳性表达率分别为2616%、6076%、3098%、1654%、0%，CINⅠ组与CINⅡ组、CINⅢ组和宫颈鳞癌组的HPV L1壳蛋白阳性表达率之间差异性均具有统计学意义（P均005）。在NILM、ASCUS、 LSIL、HSIL、SCC中，HPV L1壳蛋白的阳性表达率分别为2015%、3529%、6363%、1839%、0%，HSIL组与 LSIL组的HPV L1壳蛋白的阳性表达率之间的比较，差异有显著统计学意义（P均005）。结论：HPV L1壳蛋白的表达随宫颈病变程度的加重而降低，表明HPV L1壳蛋白表达的检测对癌前病变的筛查和预防有着重要意义。 　　【关键词】免疫组织化学法；HPV L1壳蛋白；宫颈病变 　　Relationship between the expression of HPV L1 protein shell and severity of cervical lesionsYE Qiong1， JIANG Yi1△， WANG Huan1， CHEN Liya1， ZOU Qiongyu1， KE Xiaohui2. 1.Department of Pathology， Wenzhou Central Hospital， Wenzhou 325000， Zhejiang， China； 2.Department of Obstetrics and Gynecology， Wenzhou Central Hospital， Wenzhou 325000， Zhejiang， China 　　【Abstract】Objectives： To study the relationship between the expression of HPV L1 protein shell and the severity of cervical lesions using immunohistochemical method. Methods： 529 patients in the gynecologic endocrine clinic of our hospital were chosen. Cervical exfoliated cells specimens and thin layer liquid based cytology examination were performed on all patients. Meanwhile， immunocytochemistry method was used to detect HPV L1 shell protein expression and analyze its correlation with different degrees of cervical lesions. Results： In chronic cervicitis， CINⅠ， CINⅡ， CINⅢ and cervical adenocarcinoma， the positive expression rate of HPV L1 shell protein were 26.16%， 6076%， 3098%， 16.54% and 0% respectively. Difference in HPV L1 protein positive expression rate between CINⅠ group and CINⅡ， CINⅢ and cervical adenocarcinoma was statistically significant （P005）. In NILM， ASCUS， LSIL， HSIL and SCC， the HPV L1 shell protein positive expression rate were 2015%， 35.29%， 63.63%， 18.39% and 0%， with statistically significant difference between HSIL and LSIL group （P005）. Conclusions： The expression of