Neuregulin1β对大鼠缺血再灌注后GAP43表达及神经功能影响.docVIP

Neuregulin1β对大鼠缺血再灌注后GAP43表达及神经功能影响 　　【摘要】 目的 观察神经调节蛋白-1(Neuregulin-1β;NRG-1β)对大鼠脑缺血再灌注后灰质梗死周围区生长相关蛋白(GAP-43)的影响。方法 成年健康雄性SD大鼠54只,随机分NRG-1β给药组,NS对照组,空白对照组。采用线栓法建立大脑中动脉缺血再灌注动物模型(MCAO/R),分别于MCAO/R后1 d组,3 d组和7 d组进行神经功能学评分;斜板实验:测大鼠随意运动功能及RT-PCR法观察GAP-43 m RNA的表达。结果 NRG-1β组3 d和7 d,GAP-43 mRNA表达较NS对照组和空白组表达增加,差异有统计学意义(P0.05)。NRG-1β注射后7 d,神经功能也有改善。结论 神经调节素促进大鼠脑灰质缺血再灌注后神经功能恢复,改善大鼠随意运动和提高GAP-43mRNA表达,具有神经保护作用。 　　【关键词】 神经调节蛋白-1;GAP-43蛋白;脑缺血;再灌注;大鼠 　　 　　Effects of Neuregulin-1β on GAP-43 expression and neurobihavioral improvement after cerebral ischemic reperfusion in rats 　　 　　CAI Dong-miao,ZHANG Liang-cheng,ZHOU Lin-ying,et al.Department of Anesthesiology,Affiliated Union Hospital,Fujian Medical University,Fujian 350001 Fuzhou,China 　　 　　【Abstract】 Objective To study the neuroprotective effects of neuregulin-1β(NRG-1β)after cerebral ischemic reperfusion in rats.Methods 54 adult male rats were divided randomly into NRG-1β group,NS group and sham group.Nylon Monofilament was applied to establish the middle cerebral artery occlusion reperfusion models(MCAO/R)in the rats.The rats in each group were sacrificed at 1 d,3 d and 7 d after MCAO/R.The nervous behavioral function was evaluated with Zea longa score and inclined plate experiment after the rat wake one hour.RT-PCR was used to detect the expression of GAP-43 mRNA in in the gray matter around the cerebral infarct of rats.Results The expression of GAP-43 mRNA in gray matter of rats were obviously increased in NRG-1β group compared with controlled group and sham group(P0.05).Also the nervous behavioral function had better restoration in NRG-1β group.Conclusion Neuregulin-1β may have neuroprotection by improving the nervous behavioral founction and the expression of GAP-43 mRNA in gray matter around the cerebral infarct of rats. 　　【Key words】 　　Neuregulin-1;GAP-43 protein;Brain ischemia;Reperfusion;Rats 　　 　　基金项目:福建省科技厅三项费基金资助(项目编号:2007F5054) 　　作者单位:350005福州,福建医科大学附属协和医院麻醉科(蔡东妙 张良成 郑晓燕 赖忠盟); 　　福建医科大学基础医学院病理学系电镜室(