NF―κB抑制剂Bay 11―7082对人胰腺癌细胞株SW1990增殖和凋亡影响.docVIP

NF―κB抑制剂Bay 11―7082对人胰腺癌细胞株SW1990增殖和凋亡影响 　　【摘要】 目的：观察特异性阻断NF-κB信号通路对人胰腺癌SW1990细胞增殖和凋亡的影响并探讨其可能的分子机制。方法：SW1990细胞经不同浓度（2.5 μmol/L、5 μmol/L和10 μmol/L）特异性NF-κB通路阻断剂Bay 11-7082处理后，光学显微镜和荧光显微镜下观察SW1990细胞形态学变化；cck-8法测Bay 11-7082对胰腺癌细胞生长情况的影响；流式细胞术观察细胞凋亡；western印迹检测细胞中caspase-3和Bax表达的变化。结果：Bay 11-7082可抑制胰腺癌SW1990细胞生长，其作用呈剂量依赖性；Bay 11-7082处理后凋亡胰腺癌细胞呈剂量依赖性增加；随着Bay 11-7082剂量的增加，细胞中caspase-3和Bax表达水平上调。结论：Bay 11-7082对胰腺癌细胞有一定增殖抑制和诱导细胞凋亡的作用，诱导细胞凋亡可能是其增殖抑制的主要作用方式，其机制可能为通过活化caspase-3和上调Bax的表达实现。 　　【关键词】 胰腺肿瘤； 凋亡； NF-κB 　　The Effect of NF-κB Inhibitor Bay 11-7082 on Proliferation and Apoptosis in Human Pancreatic Cancer Cell Line SW1990/ZHANG San-jun.//Medical Innovation of China，2015，12（04）：014-017 　　【Abstract】 Objective：To study the effect of blocking the NF-κB signaling pathway on proliferation and apoptosis in human pancreatic cancer cell line SW1990 and its possible mechanism.Method：SW1990 cells were treated with different concentrations of NF-κB inhibitor Bay 11-7082（2.5 μmol/L，5 μmol/L和10 μmol/L）， SW1990 cells morphological changes after treatment were observed under the optical microscope and fluorescence microscopy.CCK-8 assay was used to evaluate the growth influence of Bay 11-7082 on pancreatic cancer SW1990 cells， the flow cytometry was used to determine apoptosis in SW1990 cells.Western blot was used to detect the protein expression of caspase-3 and Bax.Result：A dose-dependent inhibitory effect of Bay 11-7082 on pancreatic cancer SW1990 cells was observed.The result of FCM indicated that treated with Bay 11-7082 exhibited a significant increase of apoptosis in a dose-dependent manner.The expression of caspase-3 and Bax was up-regulated in human pancreatic cancer SW1990 cells after treatment.Conclusion：Bay 11-7082 inhibits the proliferation of SW1990 cells， which is mediated by altering apoptosis-related genes through up-regulation of caspase-3 and Bax. 　　【Key words】 Pancreatic neoplasm； Apoptosis； NF-κB 　　First-author’s address：The First People’s Hospital of Yuey