海洋甾体对心肌梗死大鼠心功能影响及相关机制研究.docVIP

海洋甾体对心肌梗死大鼠心功能影响及相关机制研究 　　[摘要] 目的：观察海洋甾体（YC-1）对心肌梗死大鼠心功能的影响及相关的机制。方法：将72只SD大鼠随机分为6组，每组12只，即对照组、假手术组、溶剂对照组、心肌梗死组、YC1治疗组、地奥心血康治疗组。观察大鼠血浆NO与内皮素（ET-1）变化，心肌离子型谷氨酸受体（NMDANR1）的表达及大鼠心功能的变化。结果：与心肌梗死组大鼠比较，假手术组与溶剂对照组无明显变化，YC-1治疗组大鼠心功能明显改善，地奥心血康治疗组心功能明善更明显；YC-1治疗组大鼠血浆NO含量显著减少（P0.01），YC-1治疗组大鼠血浆ET-1含量显著增加（P0.01），大鼠心功能明显改善（P0.01）；YC-1治疗组大鼠心肌NMDANR1的表达明显减轻（P0.01）。结论：YC1治疗能够明显减轻心肌梗死大鼠心肌损伤，改善心功能，其作用可能与血浆NO及ET-1含量变化及NMDANR1的表达有关。 　　[关键词] 海洋甾体；心功能；心肌梗死； 改善 　　[中图分类号] R542.2 [文献标识码]A[文章编号]1673-7210（2011）07（c）-017-03 　　 　　The effects of steroid YC-1 on heart function and its underlying mechanism in cardiac infarction rats 　　GUAN Xinmin1, MENG Rongsen1,4, CHEN Siwei1, PEI Zhaohui2,3, QIU Pengxin2, YAN Guangmei3 　　1.Department of Cardiology, Guangzhou Red Cross Hospital, Guangdong Province, Guangzhou 510220, China; 2.Department of Cardiology, the Third People′s Hospital of Nanchang City, Jiangxi Province, Nanchang 330009, China; 3.Department of Pharmacology of Zhongshan Medical College, Sun Yat-sen University, Guangdong Province, Guangzhou 510800,China; 4.Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, Guangdong Province, Guangzhou 510800, China 　　[Abstract] Objective: To observe the effect of steroid YC-1 on heart function and study its underlying mechanism after acute myocardial infarction in rats. Methods:72 male Sprague Dawley (SD) rats were randomized into control group （C），sham operation group （SO），sham operation+DMSO group （OD），LAD occlusion group （LO），LO+YC-1 group （LODY）， LO＋Dia group （LODD）, 12 rats in a group respectively to measure the changes of NO，ET-1，NMDANR1 and heart function. Results: Compared with the LO group, and the heart function of rats in LODY group was evidently ameliorated (P0.05), it was more evidently ameliorated (P0.01) in LODD group. The contents of NO in rat plasma were significantly decreased in LODY group (P0.01), the contents of ET-1 in rat plasma were significantly increased (P0.01) in LODY group, the NMDANR1 e