一氧化氮对胎兔动脉导管胱硫醚―γ―裂解酶硫化氢体系影响.docVIP

一氧化氮对胎兔动脉导管胱硫醚―γ―裂解酶硫化氢体系的影响 　　摘要：目的 探讨H2S、NO对早产胎兔动脉导管的作用及其相互影响，进一步探索动脉导管开放与关闭的病理生理机制、意义及其影响因素，为临床上全面认识动脉导管的开放与关闭提供理论依据。方法 将孕21d日本大耳白兔24只随机分成四组：空白对照组及三个处理组，处理组待孕龄23d和25d时分别腹腔注射以不同剂量的一氧化氮前体-硝普钠，待孕龄26d时解剖孕兔取胎兔，胎兔心脏取血1mL后立即解剖胎兔取动脉导管组织，进行实验。结果 随着注射硝普钠浓度的增加，胎兔血浆中硫化氢含量、动脉导管组织CSEmRNA表达量均逐渐随注射剂量的增加而增加。各组间相比较，P0.01，具有显著性差异。结论 早产兔血浆中有H2S存在，早产兔动脉导管组织中存在CSEmRNA表达；给孕兔注射不同剂量硝普钠（在一定范围内）对早产兔动脉导管中H2S/CSE体系具有浓度依赖性促进作用。 　　关键词：动脉导管；早产兔；一氧化氮；硫化氢；胱硫醚-γ-裂解酶 　　Abstract： Objective To observe nitric oxide and hydrogen sulfide on ductus arteriosus in preterm fetal rabbits the role and influence each other， to further explore the pathophysiology of arterial catheter opening and closing mechanism， significance and impact factors， a comprehensive understanding of clinical arterial catheter provides a theoretical basis. Methods For Pregnant 21 days of 24 Japanese white rabbits were randomly divided into four groups： control group and three treatment groups， until 23 days and 25 days gestational age were more than intrauterine dose of sodium nitroprusside injection.Anatomy of gestational age until 26 days pregnant rabbit rabbit fetus， fetal 1mL Rabbit heart blood taken immediately after the arterial anatomy of fetal tissue in rabbits，and the experiment was conducted. Results Preterm fetal rabbits plasma hydrogen sulfide content and the expression levels of CSEmRNA， increased gradually withl injection of increasing doses of sodium nitroprusside. Conclusion Hydrogen sulfide in premature rabbit plasma exists ，the expression of CSEmRNA and CSE in preterm rabbit ductus arteriosus is higher than full-term fetal rabbit ； different doses of sodium nitroprusside （within a certain range） of the ductus arteriosus in preterm rabbits in a concentration H2S/CSE pathway dependent role in promoting. 　　Key words：Ductus arteriosus； Premature rabbits； Nitric oxide；Hydrogen sulfide； Cystathionine-γ-lyase 　　目前国外学者对动脉导管关闭分子机制的研究表明，动脉导管在宫内本身开放是处于一个主动的状态，而动脉导管自身具有一定的张力和收缩倾向，但在宫内动脉导管收缩能力被各种扩血管的因素所抑制，出生后由于扩张和收缩动脉导管的各