不同时期失面神经支配肌细胞直径与酶含量变化研究.docVIP

不同时期失面神经支配肌细胞直径与酶含量变化的研究 　　[摘要]目的：观察不同时期大鼠眼轮匝肌失神经支配后肌细胞直径与酶含量的变化，为临床面神经损伤最佳手术修复时机提供理论依据。方法：建立面神经损伤大鼠模型，应用计算机图像分析仪及比色分析技术，分别观察面神经离断术后第1、3天,第1、2、4、6、8周眼轮匝肌肌细胞直径和Ca2+-ATP酶、Na+-K+-ATP酶含量的变化。结果：面神经离断术后眼轮匝肌肌细胞直径从正常值降到只占正常值的20%；Ca2+-ATP酶、Na+-K+-ATP酶的灰度值亦分别从术后1天的（1.24±0.17）、（8.55±0.36）增加到术后8周的（1.42±0.10）、（9.74±0.33），差异均有统计学意义（P0.05）。结论：大鼠面神经离断术后眼轮匝肌肌细胞直径、Ca2+-ATP酶、Na+-K+-ATP酶含量下降，并且随失神经支配时间延长呈进行性下降，提示面神经损伤修复手术应早期进行，有利于面神经功能恢复。 　　[关键词]失神经支配；不可逆肌萎缩；肌细胞；Ca2+-ATP酶；Na+-K+-ATP酶 　　[中图分类号]R332 Q813.1 [文献标识码]A [文章编号]1008-6455（2012）03-0422-02 　　 　　Study on the the diameter and enzyme content changes of muscle cells lack of facial-nerve innnervation at different periods 　　YU Rui-fang1,ZHANG Bao-lin2,WANG Feng1,FENG Rui-zheng2 　　(1.Shanxi Medical University,Taiyuan 030001,Shanxi,China;2.Department of Plastic Surgery,The First Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China) 　　 　　Abstract: Objective We aimed at the rats orbicularis oculi muscle cell diameter and enzyme content changes at different periods of facial-nerve denervation in order to provide a theoretical basis for the best timing of facial-nerve surgical repair. Methods Rat models of facial nerve injury at different periods (1d, 3d, 1w, 2w, 4w, 6w, 8w) were made and the changes of orbicularis oculi muscle cell diameter and the Ca2+-atpases, Na+-K+-atpases content were observed by computer image analyzer and colorimetric analysis at different denervation periods. Results After facial nerve denervation, the diameter of muscle cell decreased to 20% of normal amount, and the grey level of Ca2+-atpases, Na+-K+-atpases increased from the first day after surgery（1.24±0.17）,（8.55±0.36）to the eighth week（1.42±0.10）,（9.74±0.33）. The differences were statistically significant（P0.05）. Conclusion The muscle cell diameter,the Ca2+-atpases and Na+-K+-atpases content decreased after facial-nerve denervation and have a progressive decline with the time extension, which suggests that early facial-nerve s