不同炮制方法对女贞子化学成分影响.docVIP

不同炮制方法对女贞子化学成分的影响 　　摘要：目的 考察不同炮制方法对女贞子主要化学成分特女贞苷和齐墩果酸含量的影响。方法 用同一批次女贞子进行炮制加工，采用反相高效液相色谱法，对女贞子生品、酒制女贞子、醋制女贞子和盐制女贞子中的特女贞苷和齐墩果酸进行含量测定。结果 女贞子生品及3种不同炮制品中特女贞苷含量依次为盐制女贞子（1.997 5%）女贞子生品（1.600 7%）酒制女贞子（1.144 6%）醋制女贞子（0.894 6%）。女贞子生品及3种不同炮制品中齐墩果酸含量依次为酒制女贞子（1.120 2%）盐制女贞子（0.924 6%）醋制女贞子（0.913 2%）女贞子生品（0.899 8%）。结论 女贞子不同炮制方法对其特女贞苷和齐墩果酸的含量有一定影响。 　　关键词：女贞子；特女贞苷；齐墩果酸；炮制 　　DOI：10.3969/j.issn.1005-5304.2015.07.023 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2015）07-0082-04 　　Effects of Different Methods of Preparation on the Contents of Chemical Components in Ligustri Lucidi Fructus XIAO Wei， HUANG Jan， CHEN Zhi-feng， GUO Gui-ming （Beijing Hospital of Traditional Chinese Medicine， Capital Medical University， Beijing 100010， China） 　　Abstract：Objective To investigate the effects of different methods of preparation on the contents of nuzhenide and oleanolic acid in Ligustri Lucidi Fructus. Methods The same batch of Ligustri Lucidi Fructus was under preparation and process. RP-HPLC was used to determine the contents of nuzhenide and oleanolic acid in crude samples， wine-prepared samples， vinegar-prepared samples， and salt-prepared samples. Results The contents of nuzhenide were in the order as follow：salt-prepared samples （1.997 5%）crude samples （1.600 7%）wine-prepared samples （1.144 6%）vinegar-prepared samples （0.894 6%）. The contents of oleanolic acid were in the order as follow：wine-prepared samples （1.120 2%）salt-prepared samples （0.924 6%）vinegar-prepared samples （0.913 2%）crude samples （0.899 8%）. Conclusion Different methods of preparation have certain effects on the contents of nuzhenide and oleanolic acid in Ligustri Lucidi Fructus. 　　Key words：Ligustri Lucidi Fructus；nuzhenide；oleanolic acid；method of preparation 　　基金项目：首都医科大学附属北京中医医院院级课题（2013-26） 　　通讯作者：郭桂明，E-mail：ggmjq@ 　　[2] De Feudis FV. A brief history of EGb 761? and its therapeutic uses[J]. Pharmacopsychiatry，2003，36（S1）：2-7. 　　[3] Mahadevan S， Park Y. Multifaceted therapeutic benefits of Ginkgo biloba L.：chemist