不同酸剂诱导人工沉香分析与评价.docVIP

不同酸剂诱导人工沉香的分析与评价 　　[摘要]?研究按照《中国药典》（2015年版）要求测定样品醇溶性浸出物含量，同时建立GCMS指纹图谱，使用《中药指纹图谱相似度评价（2012年版）》计算指纹图谱相似度，峰面积归一化法计算170～270 min与0～100 min 2区间总峰面积的比值，自动质谱退卷积定性系统（AMDIS）结合保留指数（RI）鉴定共有组分和差异组分，OPLSDA结合相关系数P（corr）和变量权重（VIP）筛选差异组分，灰色关联度和TOPSIS分析法综合评价人工沉香品质。结果显示18批人工沉香中15批浸出物含量符合要求，指纹图谱相似度在0439～0779，2区间总峰面积比值在0307～13254，共鉴定出9个共有组分和8个差异组分，综合上述20个指标进行灰色关联度和TOPSIS分析得到2%水杨酸诱导的人工沉香品质最优。建立的灰色关联度结合TOPSIS分析法可快速判断不同酸性诱导剂生产的人工沉香品质，为人工沉香的全面质量评价提供参考依据。 　　[关键词]人工沉香； 酸性诱导剂； 气相质谱； 灰色关联度； TOPSIS 　　Analysis and evaluation of chemically induced artificial agarwood 　　ZHOU Xin1， CHEN Liyan 1， CHEN Xiaodong1，3， ZHONG Zhaojian1， 　　LI Haohua2， ZHANG Weimin2*， GAO Xiaoxia1* 　　（1. School of Pharmacy， Guangdong Pharmaceutical University， Guangzhou 510006， China； 　　2. State Key Laboratory of Applied Microbiology Southern China， Guangdong Provincial Key Laboratory of 　　Microbial Culture Collection and Application， Guangdong Open Laboratory of Applied Microbiology， Guangdong Institute of Microbiology， Guangzhou 510070， China； 　　3. Sirio Pharm Co.， Ltd.， Shantou 515041， China） 　　[Abstract]In this study，the content of ethanol extraction of agarwood were performed following the method of Chinese Pharmacopoeia（ChP 2015 edition） The chromatographic fingerprints were established by GCMS Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine（TCM）（version 2012） was employed to calculate the similarity of each chromatogram of agarwood The ratios of sum peak area in the range of 170270 min and 0100 min of individual chromatogram were calculated using square peaks to normalization AMDIS and RI were employed to identify the common and different peaks Correlation coefficient P（corr） combined with Variable important in projection（VIP） value was employed to screen the different representative components based on OPLSDA analysis Grey related degree and TOPSIS were used to evaluate the quality of artificial agarwoods The results showed that more than 100% of the ethanol ext