与胡桃楸茎枝同煮鸡蛋中吸收成分提取工艺研究.docVIP

与胡桃楸茎枝同煮鸡蛋中吸收成分的提取工艺研究 　　【摘 要】 目的：研究胡桃楸茎枝含药鸡蛋中吸收成分的提取工艺，为胡桃楸的抗肿瘤有效成分研究奠定基础。方法：采用不同溶剂提取胡桃楸茎枝的含药鸡蛋提取液作为供试品溶液，以供试品溶液中HPLC峰个数及峰面积总和为评价指标。通过单因素试验对提取溶剂中加酸量、提取方法和提取溶剂种类进行选择，通过正交试验对提取溶剂的浓度、提取溶剂的用量和提取时间进行优化。结果：通过单因素试验确定提取溶剂中不加酸，采用回流提取，提取溶剂为含水丙酮；正交试验确定最佳提取工艺为：50倍量50%丙酮回流提取2h。结论：优选后的提取工艺稳定可靠、合理可行，为研究胡桃楸含药鸡蛋中吸收的成分奠定了基础。 　　【关键词】 胡桃楸；茎枝；含药鸡蛋；提取工艺；正交设计 　　【中图分类号】R284.2 【文献标志码】 A 【文章编号】1007-8517（2016）04-0024-05 　　Abstract：Objective Eggs cooked with the branches of Juglans mandshurica which was called drug-contained eggs. For the research of constituents absorbed by the drug-contained eggs， this study was conducted to optimize the extraction technology of the drug-contained eggs.Methods For the optimization of acid content added into the extracting solvent， extracting method， and extracting solvent， single factor experiment was designed. Orthogonal experiment was conducted to choose the time of extraction， concentration and volume of the extracting solvent. The total number and the sum of area of chromatographic peak were taken as investigation index.Results The result of single factor experiment demonstrated that acetone and reflux was suitable for the extraction and no acid should be added into the extracting solvent. Orthogonal experiment suggested that the drug-contained eggs should be extracted with 50 times of 50% acetone by reflux for 2 hours. Conclusions The optimum extracting technology is reasonable and stable. This work was useful for the research of the constituents absorbed by the drug-contained eggs. 　　Keywords：Juglans mandshurica；Branches；Drug-contained eggs；Extraction Technology；Orthogonal Design 　　胡桃楸始载于《开宝本草》，具有补肺益肾、滋阴助阳、抗癌的功效[1]。民间将胡桃楸与鸡蛋同煮用于治疗肿瘤[2]，轻者吃鸡蛋，重者蛋汤同吃。本课题组前期对与胡桃楸不同部位同煮的鸡蛋（含药鸡蛋）进行抗肿瘤活性研究，发现与胡桃楸不同部位同煮的含药鸡蛋均有不同程度的抗肿瘤作用，其中，茎枝的含药鸡蛋抗肿瘤作用最明显。但尚未发现有关提取含药鸡蛋吸收成分的研究报道。另外，含药鸡蛋中吸收的成分较多，不能以单一成分或某类成分的含量为考察指标，为了全面准确的分析含药鸡蛋中吸收的成分，研究以HPLC图谱中峰个数及峰面积总和为指标，采用单因素试验和正交试验相结合，对与胡桃楸茎枝同煮的鸡蛋中吸收成分的提取工艺进行了优化，为深入研究胡桃楸含药鸡蛋的抗肿瘤作用及胡桃楸的合理开发利用奠定基础。 　　1 仪器与材料 　　1.1 仪器 Agilent 1260高