十精丸对UVA致ESF1细胞光老化保护作用研究.docVIP

十精丸对UVA致ESF1细胞光老化保护作用研究 　　[摘要]目的：研究十精丸抗紫外线致ESF-1细胞光老化作用，并初步探讨其作用机制。方法：用40J/cm2的UVA照射人皮肤成纤维细胞建立光老化细胞模型，以不同浓度十精丸提取物处理光老化细胞，MTT法检测细胞活力，羟胺法检测细胞超氧化物歧化酶（SOD）活性，比色法检测谷胱甘肽过氧化物酶（GSH-Px）活性，TBA法检测丙二醛（MDA）含量。结果：UVA照射成纤维细胞后，细胞活力下降，SOD、GSH-Px活性降低，MDA含量升高（P0.01），十精丸水提液中（100μg/ml）、高（200μg/ml）剂量组能显著提高成纤维细胞细胞活力、SOD、GSH-Px活性，降低MDA含量（P0.05或P0.01）。结论：十精丸水提液可抑制UVA引起的成纤维细胞活性下降，推测其机制为通过提高SOD活力、加速氧自由基的清除和减少氧自由基的产生，使细胞的脂质过氧化损伤程度降低有关。 　　[关键词]十精丸；UVA；ESF-1细胞；光老化 　　[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455（2011）04-0582-03 　　 　　Protective effect of Shijingwan on photoaging induced by Ultraviolet A in ESF-1 cell 　　CHEN Li-juan,WANG Jia-zhi,XU Jiao,QI Yong-hua,HUANG Xin-hong 　　(Department of Cosmetology,Jiamusi College, Heilongjiang University of Traditional ChineseMedicine,Jiamusi 154007,Heilongjiang,China) 　　 　　Abstract: Objective To study the protective effect of Shijingwan agains UV irradiation-induceddamage in ESF-1 cells and its mechanism. Methods The model of photoaging fibroblasts was established by irradiating of ultraviolet light(40J/cm2). The different concentration water-extract solution from Shijingwan were used for photoaging cell. The proliferation of human fibroblasts was detected by MTT, and superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activity, malondialdehyde(MDA) content were assayed by hydroxylamine, colorimetric and TBA methods respectively. Results The proliferation activity of fibroblasts descended with the UVA irradiation, the activities of SOD and GSH-Px decreased, when the content of MDA was ascensive(P0.01). But under the intervention at middle concentration(100μg/ml) and high concentration(200μg/ml) of water-extract solution from Shijingwan,the above changes induced by UVA irradiation were inhibited(P0.05 or P0.01). Conclusion The water-extract solution from Shijingwan might inhibit the proliferation decreasing which induced by UVA. And its mechanism is due to its abilities of increasing SOD，scavenging oxygen free radicals. 　　Keywords:Shijingwan