同种异体骨髓基质干细胞移植对兔激素性股骨头缺血性坏死血清肿瘤坏死因子α影响.docVIP

同种异体骨髓基质干细胞移植对兔激素性股骨头缺血性坏死血清肿瘤坏死因子α影响 　　【摘要】 目的 研究同种异体骨髓基质干细胞(MSC)移植对兔激素性股骨头缺血性坏死(SANFH)血清肿瘤坏死因子α(TNF-α)的影响。方法 将45只健康新西兰兔随机分为三组:空白组、每组各15只。模型组和治疗组,模型组和治疗组,第1、3周马血清10 ml/kg耳缘静脉注射,于第5周腹腔注射醋酸泼尼松龙7.5 mg/kg,1次/d,连续3 d;空白组,第1、3周生理盐水10 ml/kg兔耳缘静脉注射,于第5周腹腔注射生理盐水1 ml,1次/d,连续3 d。治疗组于第6周行MSC移植。第10周测血清中TNF-α水平。结果 治疗组兔血清TNF-α水平较模型组明显偏低( P0.01)。结论 MSC移植使兔SANFH模型血清TNF-α水平降低。 　　【关键词】 骨髓基质干细胞;股骨头坏死;肿瘤坏死因子 　　 　　Effects of allogenic marrow stromal cells transplantation on the TNF-α in rabbits with steroid-induced avascular necrosis of femoral head 　　 　　CHEN Xuan-wei, LIN Wen-ping, LIN Jian-hua. 　　Department of Orthopaedics, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China 　　 　　【Abstract】 Objective To study the effects of allogenic marrow stromal cells transplantation on the TNF-α in rabbits with steroid-induced avascular necrosis of femoral head. Methods Forty-five healthy New Zealand white rabbits were randomly divided into control group,model group and treatment group with 15 in each group.Model group and treatment group were injected with horse serum and prednisone.The model group was given hormal saline treatment group was transplanted of allogenic marrow stromal cells at 6 weeks.The level of TNF-α in serum of the rabbits of the three groups was examined at 10 weeks.Results The level of TNF-α in rabbits serum of treatment group was lower than that of model group (P0.01).Conclusions Marrow stromal cells transplantation can decrease the level of TNF-α on steroid-induced avascular necrosis of femoral head in rabbits. 　　【Key words】 Marrow stromal cells(MSC); Femoral head necrosis;Tumor necrosis factor-α 　　 　　基金项目:福建省科技计划项目(项目编号:2005y014) 　　作者单位:350001 福建医科大学附属第一医院骨科 　　 　　随着糖皮质激素的大量应用,股骨头缺血坏死(Avascular necrosis of the femoral head,ANFH)的发病率逐年增高,发病年龄也趋于年轻化,常发生于30～50岁的中青年[1-2],而临床上尚无满意的治疗方法。组织工程学的进展使骨髓基质干细胞(Marrow stromal cells, MSC)的理论与临床应用研究越来越受到重视。本研究采用体外扩增培养的MSC移植治疗兔激素性股骨头缺血性坏死( steroid-induced avascular necrosis of femoral head,SANFH),