基于多元统计分析5种海洋贝壳类中药微量元素比较研究.docVIP

基于多元统计分析5种海洋贝壳类中药微量元素比较研究 　　[摘要] 该论文比较研究中国药典收载5种海洋贝壳类中药的微量元素含量差异，并探讨微量元素与功效的相关性。采用电感耦合等离子体质谱法，测定51批次贝壳饮片中的钴、铜、锌、钼、硒、砷和钡等7种微量元素含量，利用主成分分析（principal component analysis，PCA）等多元统计方法分析数据，发现硒元素的含量在上述贝壳类中药同品种不同批次饮片中的差异性较小，但在不同品种间有显著性差异，可作为区分不同品种贝壳类中药的关键元素之一。此外，对同一中药品种来说，在不同批次饮片中含量差异较小的无机元素还有：石决明中钴（Co）、铜（Cu）、钼（Mo）和钡（Ba），珍珠母中Co和砷（As），瓦楞子中Mo，As和Ba，蛤壳中Zn，提示以上微量元素可以作为该生药质量控制的潜在化学指标。从PCA得分散点图可见，瓦楞子和蛤壳能归为一大类，牡蛎、石决明和珍珠母归为另一大类。已知前两者是化痰药，后三者属于平肝潜阳药，这提示贝壳类中药所含的微量元素与其功效有一定相关性。PCA分析所得因子载荷矩阵提示，硒元素可能是导致贝壳类中药功效差异的重要化学基础。该文通过对5种海洋贝壳类中药51批次饮片的微量元素比较分析，寻找该类药材不同品种间的差异性，为该类中药质量标准的制定提供科学依据。 　　[关键词] 贝壳类中药；微量元素；电感耦合等离子体质谱；主成分分析；多元统计分析 　　[Abstract] A comparable study were carried out by determination of trace elements on five marine-derived shell traditional Chinese medicine（TCM）（Ostreae Concha， Haliotidis Concha， Margaritifera Concha， Meretricis Concha， and Arcae Concha）， which were recorded in the Chinese Pharmacopoeia （2010 version）. Seven trace elements in 51 batches of this type of shell TCM were analyzed by Inductively Coupled Plasma Mass Spectrometry （ICP-MS）， combined with principal component analysis （PCA） methods. The content of element Se， which exhibited significant differences among different drugs， could be used as a key element to distinguish this type of drugs. Meanwhile， the contents of elements Co， Cu， Mo， and Ba in Haliotidis Concha， Co and As in Margaritifera Concha， Mo and As in Meretricis Concha， Mo， As， and Ba in Arcae Concha， and Zn in Meretricis Concha were relatively stable. In the PCA plot， Arcae Concha and Meretricis Concha could be efficiently distinguished from Ostreae Concha together with Haliotidis Concha， and Margaritifera Concha. The results also showed a correlation with their medicinal function. In conclusion， trace elements in marine-derived shell TCM could not be neglected for their quality control. 　　[Key words] marine-derived shell traditional Chinese medicine； trace element； inductively coupled plasma mass spectrometry； principal component analysis； mu