复方钩藤降压片对自发性高血压大鼠血管重塑单核细胞趋化蛋白―1和基质金属蛋白酶―9表达影响.docVIP

复方钩藤降压片对自发性高血压大鼠血管重塑单核细胞趋化蛋白―1和基质金属蛋白酶―9表达影响 　　摘要：目的 观察复方钩藤降压片对自发性高血压大鼠（SHR）血管重塑单核细胞趋化蛋白-1（MCP-1）、基质金属蛋白酶-9（MMP-9）表达的影响，探讨其可能的作用机制。方法 24只12周龄雄性SHR随机分为模型组、复方钩藤降压片组和阳性药组，每组8只，另设8只WKY大鼠为空白对照组。各给药组给予相应药物灌胃，连续6周。无创尾动脉测压法观测血压；HE染色观察大鼠胸主动脉、肾动脉形态学变化；免疫组化检测胸主动脉内皮细胞MCP-1、MMP-9的蛋白表达。结果 模型组胸主动脉壁MCP-1、MMP-9蛋白表达明显高于空白对照组（P0.01）；与模型组比较，各给药组胸主动脉壁MCP-1、MMP-9蛋白表达显著降低（P0.05，P0.01）；复方钩藤降压片组与阳性药组比较，胸主动脉壁内皮细胞MCP-1、MMP-9蛋白表达无明显差异。结论 复方钩藤降压片具有降低SHR血压，减轻炎症反应，调控血管重塑的作用，其机制可能与下调SHR胸主动脉壁内皮细胞MCP-1、MMP-9蛋白表达有关。 　　关键词：复方钩藤降压片；自发性高血压大鼠；血管重塑；单核细胞趋化蛋白-1；基质金属蛋白酶-9 　　DOI：10.3969/j.issn.1005-5304.2016.10.013 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）10-0051-05 　　Abstract： Objective To observe the effects of Compound Uncaria Hypotensive Tablets on expressions of MCP-1 and MMP-9 of vascular remodeling in spontaneously hypertensive rats （SHR）； To discuss it possible mechanism of action. Methods Totally 24 12-week old male SHR were randomly divided into SHR model group， Compound Uncaria Hypotensive Tablets group， and positive medicine group， with 8 rats in each group. Another 8 WKY rats were set as normal control group. Medication groups were given relevant medicine for gavage for successive 6 weeks. Noninvasive tail cuff method was used to observe blood pressure； morphological changes in thoracic aorta and renal artery were observed by HE staining； immunohistochemistry was used to detect the protein expressions of MCP-1 and MMP-9 in thoracic aortic wall. Results Protein expressions of MCP-1 and MMP-9 in thoracic aortic wall of SHR model group were significantly higher than those of normal control group （P0.01）； Compared with the SHR model group， protein expressions of MCP-1 and MMP-9 in thoracic aortic wall decreased significantly in the medication groups （P0.05， P0.01）； Compared the Compound Uncaria Hypotensive Tablets group and positive medicine 　　高血压病是一种由多种病因相互作用导致的、复杂的、进行性的心血管综合征。近年来，炎症因素在高血压病的发生、发展及转归中的作用受到关注。有研究认为，高血压病是一个慢性炎症过程，可以产生炎症因子，影响血压水平，损伤血管内皮功能，加速血管重塑。前期研究表明，复方钩藤降压片具有降低血压，逆转心脏、肾脏的超微结