复发性翼状胬肉中VEGF表达情况临床研究.docVIP

复发性翼状胬肉中VEGF表达情况临床研究 　　【摘要】 目的：探讨VEGF在原发性翼状胬肉组织、复发性翼状胬肉组织和正常球结膜组织中的表达情况。方法：选取2015年4月-2016年4月无锡第二人民医院眼科的翼状胬肉患者胬肉病理组织共40例作为试验组，其中原发性翼状胬肉30例，复发性翼状胬肉10例，另选取正常球结膜组织10例作为对照组。采用HE染色的方法观察正常球结膜、原发性翼状胬肉患者和复发性翼状胬肉患者的组织学形态，比较三种组织形态差异。采用免疫组化方法对各组标本进行染色，利用IPP图像软件系统测定标本中VEGF表达情况的平均光密度值，采用SPSS 17.0软件进行统计分析。结果：30例原发性翼状胬肉患者的VEGF表达值为（0.0082±0.0004），10例正常球结膜患者VEGF表达值为（0.0037±0.0016），10例复发组翼状胬肉患者VEGF表达值为（0.0232±0.0014），将三组测量结果通过非参数秩和检验比较，差异有统计学意义（F=240.211，P0.01）。复发组翼状胬肉患者VEGF表达值比原发组翼状胬肉患者增高，差异有统计学意义（P0.01），原发组翼状胬肉VEGF表达值与正常组VEGF表达值相比增高，差异有统计学意义（P0.01）。结论：VEGF在复发组翼状胬肉患者中高表达，表明VEGF参与了翼状胬肉疾病的复发过程。 　　【关键词】 复发性翼状胬肉； 血管内皮生长因子 　　【Abstract】 Objective：To investigate the expression of VEGF in primary pterygium，recurrent pterygium and normal conjunctiva.Method：From April 2015 to April 2016，40 cases of pterygium in Wuxi NO.2 People’s Hospital were selected as the experimental group，including 30 cases of primary pterygium，10 cases of recurrent pterygium，another 10 cases of normal conjunctival tissue were selected as control group.HE staining was used to observe the histological morphology of normal conjunctiva，primary pterygium patients and recurrent pterygium patients，and to compare the differences between the three groups.The samples were stained with immunohistochemical method. The average light density of VEGF expression was measured by IPP image software system， and the SPSS 17 software was used for statistical analysis.Result：30 cases of primary pterygium was VEGF expression value of（0.0082±0.0004），10 cases of normal conjunctiva in patients with VEGF expression value of（0.0037±0.0016），10 cases of recurrence of pterygium patients the expression of VEGF was（0.0232±0.0014），three groups of measurement results by nonparametric rank sum test，the difference was statistically significant（F=240.211，P0.01）.The expression of VEGF in patients with pterygium recurrence group than primary pterygium were significantly increased，the difference was statistically significant（P0.01），primary pterygium group