大鼠性激素水平在邻苯二甲酸酯类化合物作用下变化.docVIP

大鼠性激素水平在邻苯二甲酸酯类化合物作用下变化 　　【摘 要】 目的：通过动物实验，研究不同剂量及不同疗程的DEHP处理后各大鼠性激素水平的改变，探讨其对大鼠卵巢功能的影响。方法：选取SD雌性大鼠80只，随机分为以下8组（每组10只）：短疗程空白对照、低、中、高剂量组及长疗程空白对照、低、中、高剂量组。低、中、高剂量组根据体重，分别灌喂DEHP：1000mg/（kg?d），2000mg/（kg?d）和3000mg/（kg?d）。空白对照组：无药物干预。短疗程灌胃14天，长疗程灌胃28天。整个观察期间，每周一、四测量大鼠体重以观察动物生长情况并调整用药剂量。观察期末，各组大鼠选用下腔静脉采血，按酶联免疫试剂盒说明书测血清中孕酮、雌二醇、睾酮、卵泡刺激素、黄体生成素含量。结果：短疗程高、中、低剂量组和空白对照组四组的睾酮水平逐渐下降（P0.05），不具有时间依赖性。与对照组相比，给药组的血清雌二醇、孕酮、卵泡刺激素及黄体生成素水平均无显著性差异（P0.05），各剂量组的长、短疗程之间的雌二醇、孕酮、卵泡刺激素及黄体生成素水平均无统计学意义（P0.05）。结论：DEHP引起大鼠血清睾酮水平上升，抑制排卵，具有生殖毒性作用，并具有剂量依赖性，但不具有明显的时间依赖性；推测DEHP对大鼠卵巢功能的影响可能是通过干扰卵巢内分泌调节功能。 　　【关键词】 邻苯二甲酸酯 邻苯二甲酸二乙基己酯 大鼠 性激素 　　【Abstract】 Objective： To evaluate the toxicity of PAEs on the reproduction-endocrine system and potential hazards to the women health， DEHP was taken as the tested chemical of this study， its effects on the hormones of female rats were examined. Methods： 80 healthy female SD rats were randomly divided into 8 groups （n = 10 each）： normal control group， low-dose group， middle-dose group， and high-dose group of short period， normal control group， low-dose group， middle-dose group and high-dose group of long period. According to body weight， low-dose， middle-dose and high-dose groups were administrated with DEHP： 1000mg/（kg?d）， 2000mg/（kg?d） and 3000mg/（kg?d） respectively. Normal control group： no drug intervention. Short period-group were administrated with DEHP for 14 days， and long period-group were administrated for 28 days. Throughout the observed period， body weight was measured on every Monday and Thursday to observe rat’s growth pattern and adjust the dosage. On the day of necropsy， all animals were exsanguinated from the inferior vena cava. And according to instructions of ELISA kit， the levels of the serum P， E2， T， FSH and LH were determined. Result： The high-dose， middle-dose， low-dose， and normal control group of short period showed a gradual decline in the levels of serum T （P 0.05）， it meant that it didn’t had a time-dependent manner. There were no signifi