大黄素对海人酸致痫小鼠海马iNOS和COX―2表达影响.docVIP

大黄素对海人酸致痫小鼠海马iNOS和COX―2表达影响 　　[摘要]目的 探讨大黄素对海人酸诱导的小鼠癫痫持续状态后海马组织诱导型一氧化合酶（iNOS）、环氧化酶-2（COX-2）表达的影响。方法 选取45只ICR雄性小鼠，随机分为对照组、癫痫持续状态组、大黄素治疗组，每组15只。采用海人酸建立小鼠癫痫模型，大黄素治疗组腹腔注射200 mg/kg的大黄素。癫痫持续状态组和对照组注入等量的生理盐水。致痫24 h后通过苏木精-伊红（HE）染色观察海马神经细胞的形态学变化，采用RT-PCR、Western blot分别检测iNOS、COX-2 mRNA与蛋白的表达。结果 大黄素可明显改善癫痫鼠海马组织的病理形态学变化；大黄素治疗组iNOS、COX-2 mRNA与蛋白的含量明显低于癫痫持续状态组，差异有统计学意义（P0.05）。结论 大黄素能降低小鼠癫痫持续状态后海马组织iNOS、COX-2 mRNA与蛋白的表达。 　　[关键词]大黄素；癫痫；iNOS；COX-2 　　[中图分类号] R742.1 [文献标识码] A [文章编号] 1674-4721（2018）1（b）-0011-04 　　[Abstract]Objective To investigate Emodin′s influence on the expression of iNOS，COX-2 in hippocampus of mice after status epilepticus by kainic acid.Methods 45 ICR male mice were selected and randomly divided into the control group，the SE group and the Emodin group，15 cases in each group.The epileptic model of mice was established by kainic acid.The treatment group was injected with 200 mg/kg by Emodin.The SE group and the control group were injected an equal amount of saline.HE stain was observed the morphological changes in hippocampal nerve after 24 h，RT-PCR and Western blot was used to detect the expression of the mRNA and protein level of iNOS，COX-2.Results Emodin could significantly relieve the hippocampal injury after status epilepticus in mice.The content of iNOS，COX-2 mRNA and protein in the Emodin group was significantly lower than that of the mRNA and protein in the SE group，with significant difference （P0.05）.Conclusion Emodin may derease the expressions of the mRNA and protein 1evel of iNOS，COX-2 in hippocampus after status epilepticus in mice. 　　[Key words]Emodin；Epilepsy；iNOS；COX-2 　　癫痫是神经科的常见疾病之一，发作形式多样，具有很高的发病率和致残率。癫痫发作可激活神经元、神经胶质细胞等释放大量氧自由基和炎症因子，从而造成脑组织的再次损伤[1]。iNOS和COX-2是神经炎症的重要介质，在缺血、缺氧等损伤因素的作用下，参与脑组织早期炎症反应，并对神经兴奋性的增高起到重要作用[2-3]。本实验采用海人酸建立癫痫模型，探讨大黄素对小鼠癫痫发作后海马组织中iNOS、COX-2表达的影响。 　　1材料与方法 　　1.1材料 　　海人酸（批号：K0250）与大黄素（批号：E7881）均购自美国Sigma公司；兔抗鼠iNOS、COX-2多克隆抗体购自Santa Cruz公司；RT-PCR试剂盒来源于Promega公司；蛋白浓度试剂盒由碧云天生物技术研究所提供；ICR健康雄性小鼠45只[许可证号：SCXK（沪）2007-0005]，体