姜黄素对铜绿假单胞菌诱导人肺上皮细胞产生细胞因子影响及其分子机制研究.docVIP

姜黄素对铜绿假单胞菌诱导人肺上皮细胞产生细胞因子影响及其分子机制研究 　　南华大学附属第三医院感染科，湖南衡阳 421000 　　[摘要] 目的 探讨姜黄素对铜绿假单胞菌（pseudomonas aeruginosa，Pa）感染的肺上皮细胞的体外抗炎效应。 方法 体外培养肺上皮细胞系NCI-H292，加入姜黄素和培养的Pa感染不同时间。Western blot检测和实时定量PCR分别检测NCI-H292细胞中红素氧合酶1（Heme oxygenase-1，HO-1）mRNA和蛋白表达情况；ELISA检测IL-1β和IL-8的产生。同时采用RNA干扰HO-1表达后，观察对Pa诱导IL-1β和IL-8产生的影响。 结果 Pa感染NCI-H292细胞后，IL-1β和IL-8含量分别为（87.52±7.96）pg/mL和（205.63±34.25）pg/mL，50 μg/mL姜黄素孵育8 h后，其含量分别降至（15.78±10.74）pg/mL和（49.82±14.28）pg/mL，处理前后差异有统计学意义（P 0.05）。同时，姜黄素能在转录翻译水平分别调控HO-1 mRNA和蛋白表达，其中50 μg/mL姜黄素能将HO-1 mRNA增高19倍，蛋白表达水平提高4.8倍（P 0.05）。HO-1 siRNA沉默HO-1表达后，与Pa组相比，IL-1β和IL-8分泌进一步增高。 结论 姜黄素可能通过上调HO-1的表达而发挥对肺上皮细胞的抗炎作用。 　　[关键词] 血红素氧合酶-1；姜黄素；铜绿假单胞菌；肺上皮细胞；白介素-1β；白介素-8 　　[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-7210（2013）11（b）-0013-04 　　Study of molecular mechanism of the effect of Curcumin on pseudomonas aeruginosa-induced cytokines production in lung epithelial cells 　　LI Bo LUO Jinhua HE Zhiguo 　　Department of Infectious Diseases， the Third Affiliated Hospital， University of South China， Hu′nan Province， Hengyang 421000， China 　　[Abstract] Objective To investigated the anti-inflammatory effects of Curcumin in pseudomonas aeruginosa -infected lung epithelial cells in vitro. Methods Lung epithelial cell line NCI-H292 was cultured in vitro， and was incubated with Curcumin and pseudomonas aeruginosa for various times. Expression of Heme oxygenase-1 （HO-1） mRNA and protein was detected by real-time PCR and Western blot， respectively； production of IL-8 and IL-8 was detected by ELISA. RNA interference was used to silent HO-1 expression， and pseudomonas aeruginosa-induced production of IL-1β and IL-8 were detected. Results After pseudomonas aeruginosa infection， the concentration of IL-1β and IL-8 was （87.52±7.96） pg/mL and （205.63±34.25） pg/mL， respectively. When incubated with 50 μg/mL Curcumin for 8 h， IL-1β and IL-8 decreased to （15.78±10.74） pg/mL and （49.82±14.28） pg/mL （P 0.05）. Curcumin could also induce NCI-H292 cells expression of HO-1 mRNA at the trans