姜黄素抑制小鼠生殖器官氧化产物生成研究.docVIP

姜黄素抑制小鼠生殖器官氧化产物生成研究 　　【摘要】目的：探讨姜黄素对氧化应激小鼠子宫、卵巢组织脂质过氧化水平的影响。方法： 昆明种雌性成年小鼠40只，随机分成正常对照组，0 mg/kg Cur组，100 mg/kg Cur组，200 mg/kg Cur组，连续用药21天，实验结束后测定小鼠子宫、卵巢组织以及血清中丙二醛（MDA）、活性氧（ROS）的含量。结果：100 mg/kg Cur组，200 mg/kg Cur组血清、子宫以及卵巢氧化应激产物ROS、MDA与砷暴露组相比均有降低，具有明显统计学差异（P0.05）。结论 ：姜黄素可降低小鼠子宫、卵巢组织以及血清中氧化产物MDA、ROS的含量，对小鼠卵巢氧化应激引起的生殖器官损伤有保护作用。 　　【关键词】姜黄素；氧化应激；卵巢；子宫；小鼠 　　Inhibit effect of curcumin on lipid peroxidation product in the reproductive organs of miceWANG Xiaoning， ZHANG Changjun， ZHANG Ying， DIAO Honglu. Center of Reproductive Medicine， Affiliated People Hospital of Hubei Medical College， Shiyan 442000， China 　　【Abstract】Objectives： To observe the different effect of curcumin on the markers of oxidative stress to explore the pathogenesis to offer more experimental evidence for the clinical use of cur cumin on the oxidative stress related ovarian diseases. Methods： 40 Kunming mice with two regular estrus cycles， without coitus and body-matured female were selected as research objects and were randomly divided into four groups， including control group， 0mg/kg Cur group， 100mg/kg Cur group， 200mg/kg Cur group. All rats were treated for 21days and examined by ELISA for the concentration of Reactive Oxygen Species （ROS）， Malondialdehyde （MDA） in serum， uterus and ovaries. Results： Compared with the 0mg/kg Cur， the concentrations of ROS and MDA in the serum， uterus and ovaries of 100mg/kg Cur group and 200mg/kg Cur group were decreased， the difference was significant （P0.05）.Conclusions： Curcumin can reduce the concentration of ROS， MDA. We conclude that curcumin can scavenge free radicals in mouse reproductive system. From this way， curcumin has good antioxidant capacity in mouse reproductive sysyterm. 　　【Key words】Curcumin； Oxidative stress； Ovary； Uterus； Mouse 　　【中图分类号】S857.17【文献标志码】A 　　氧化应激起源于反应性的氧化物与生物抗氧化剂间平衡的破坏，造成活性氧浓度升高。而高浓度活性氧会破坏细胞的DNA、蛋白质和脂类，致使细胞基因、结构及功能受损，甚至导致细胞死亡[1]。许多不同的生殖状态都存在氧化应激，如子宫内膜异位症、卵子发生、卵母细胞成熟、输卵管积水、弱精症、死精症及精子 DNA 损伤等[2]。姜黄素（Curcumin， Cur）是从姜黄属中药莪术、姜黄、