实验性鼠蛛网膜下腔出血后CSF中PICPPⅢNP动态变化及意义.docVIP

实验性鼠蛛网膜下腔出血后CSF中PICPPⅢNP动态变化及意义 　　【摘要】目的：通过检测大鼠蛛网膜下腔出血（SAH）后脑脊液中（CSF）Ⅰ型前胶原羧基端肽(PICP)、Ⅲ型前胶原氨基端肽(PⅢNP)浓度的动态变化，研究其反映蛛网膜下腔纤维化的意义。方法：采取在枕大池两次注血法建立大鼠SAH模型；在第三天、第六天、第十天、第十四天、第二十一天取脑脊液检测转化生长因子-β1（TGF-β1）和PICP、PⅢNP浓度。结果：（1）实验组脑脊液的TGF-β1较对照组明显升高（P0.01），并呈双时相；（2）实验组脑脊液的PICP、PⅢNP较对照组明显升高（P0.01）。结论：大鼠SAH发生后，脑脊液中的PICP、PⅢNP浓度升高提示蛛网膜下腔出现纤维化，早期检测可预测蛛网膜下腔的纤维化的发生。 　　【关键词】蛛网膜下腔出血；Ⅰ型前胶原羧基端肽；Ⅲ型前胶原氨基端肽；转化生长因子-β1；纤维化 　　文章编号：1009-5519（2008）07-0949-02 中图分类号：R6 文献标识码：A 　　On the change and their significance of the levels of PICP and PⅢNP in CSF of rats after SAH 　　LI Lin-yong，LIU Fei，YAN Hui，et al. 　　（Department of Neurosurgery，The Third Xiangya Hospital，Zhongnan University，Changsha 410013，China） 　　【Abstract】Objective：To research the significance ofPICP and PⅢNP reflecting the fibrosis of cavitas subarachnoidealis by detecting the concentrations of PICP and PⅢNP in cerebrospinal fluid(CSF) of rats after subarachnoid hemorrhage(SAH). Methods：The animal model of rats with SAH was made by injecting autoblood solution into cistorna magna twice. At the third day、the 6th day、the 10th day、the 14th day、the 21th day，CSF was collected and was measured the levels of TGF-1 and PICP、PⅢNP. Results：（1）The level of TGF-β1 in CSF of rats with SAH was statistically significantly greater compared with control level(P0.01).（2）The levels of PICP and PⅢNP in CSF of rats with SAH were statistically significantly greater compared with control levels(P0.01). Conclusion：After SAH，it is suggested the fibrosis of cavitas subarachnoidealis that the concentrations of PICP and PⅢNP in CSF rise significantly.And early detection of the levels ofPICP and PⅢNP in CSF may help in the prediction of the fibrosis of cavitas subarachnoidealis. 　　【Key words】Subarachnoid hemorrhage(SAH)；Carboxyterminal propeptide of type I procollagen(PICP)；Aminoterminal propeptide of type Ⅲ procollagen(PⅢNP)；Transforming growth factor-β1(TGF-β1)；Fibrosis 　　 　　蛛网膜下腔出血（subarachnoid hemorrhage，SAH）可引起蛛网膜下腔纤维化，引起脑脊液的循环障碍和吸收障碍，导致继发性交通性脑积水。Ⅰ型前胶