TrkB及SliTrk5在膀胱癌中的表达-外科学专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要 ·············································································1 英文摘要 ·············································································3 英文缩写 ·············································································6 研究论文 TrkB 及 SliTrk5 在膀胱癌中的表达 前言 ·············································································7 材料与方法 ····································································9 结果 ·············································································12 附图 ·············································································15 附表 ·············································································16 讨论 ·············································································18 结论 ·············································································21 参考文献 ·······································································22 综述 膀胱癌的检测：基于尿液和血液标志物检测的评估 ··············24 致谢 ···················································································53 个人简历 ·············································································54 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 TrkB 及 SliTrk5 在膀胱癌中的表达 摘 要 目的：膀胱癌是全世界最普遍的癌症之一，发病率正在逐年上升。膀 胱癌的发生机制非常复杂，尽管有大量研究，但有很多不明确的问题。但 如果及早检测到，膀胱癌治疗和应对会非常成功。因此，寻找膀胱癌相关 的分子，探讨作用机制，有助于我们丰富对膀胱癌的治疗和干预。 脑源性神经营养因子(BDNF)是神经营养素超家族一员，目前在神经 系统的病理生理学、心理学和神经病学的研究中，都体现出重要的功能。 最近，一些研究表明，脑源性神经营养因子及其受体 TrkB 可能参与很多 恶性肿瘤的生长和转移，如神经母细胞瘤、前列腺癌、胰腺导管癌、肝细 胞癌和肺癌等。然而目前在膀胱癌中 TrkB 是否激活尚不清楚。 Slit-和 NTRK-like 家族（SliTrks）的成员是 1 型跨膜蛋白,这种蛋白在 中枢神经系统的突触中具有的本地化和功能化的作用，在这个系统中，它 们的胞外结构与突触前结合通过跨突触膜反应来调控突触的形成。Slitrk 蛋白家族被认为与神经精神障碍有关。其中 SliTrk5 作为 TrkB 的共受体， 它调节其再循环来促进 BDNF 依赖的信号转导。通过与 TrkB 的受体直接 作用来调节脑源性神经营养因子（BDNF）依赖的生物反应过程。但 SliTrk5 是否同样在肿瘤中有异常的表达，仍未为人知。因此，本文初步研究 TrkB 和 SliTrk5 在膀胱癌组织中的表达，探讨 TrkB 和 SliTrk5 在人的膀胱癌组 织中的表达和意义，以期为膀胱癌的检测和治疗提供新的靶标。 方法：收集在华北油田总医院手术患者中的膀胱癌组织和正常膀胱组 织，应用免疫组织化学法检测正常膀胱组织和膀胱癌组织 TrkB 和 SliTrk5 蛋白表达情况，并进一步观察其表达与各种病理参数的差异性。应用 SPSS13.