VDAC与HPV16E7蛋白在CIN到宫颈癌过程中的作用-妇产科学专业论文.docxVIP

独创性声明 本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研 究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其 他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集 体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切 法律后果。 学位论文作者签名： 日期： 年 月 日 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有 权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和 借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据 库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文 本论文属于 保密□ ，在 年解密后适用本授权书。 不保密□。 （请在以上方框内打“√” ） 学位论文作者签名： 指导教师签名： 日期： 年 月 日 日期： 年 月 日 目 录 第一部分 ······················································ 1 中英文缩略词表 ·································································1 中文摘要 ··········································································2 英文摘要 ··········································································3 前言 ················································································5 实验材料 ··········································································7 实验方法 ········································································ 12 实验结果 ········································································ 24 讨论 ·············································································· 29 第二部分 ·····················································32 综述 ·············································································· 32 参考文献 ········································································ 38 附录 ·············································································· 44 致谢 ·············································································· 45 华 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE 10 PAGE 10 第一部分 中英文缩略词表 缩略词 英文全称 中文名称 HPV Human Papillomavirus 人乳头状瘤病毒 IHC Immunohistochemistry 免疫组织化学 WB Western Blot 蛋白免疫印迹法 qRT-PCR quantificational Real-time-Polymerase Chain Reaction 定量聚合酶链反应 CIN Cervical Intraepithelial Neoplasia 宫颈上皮内瘤变 IOD Integrated Optical Density 积分光密度值 Cas Caspase 半胱天冬氨酸蛋白酶 mPTP mitochondrial Permeability Transition pore 线粒体通透性转换孔 VDAC1 Voltage-dependent anion-selective channel protein1 电压依赖的阴离子通道