TESTIN基因在子宫内膜癌病程中的作用及其机制研究-妇产科学专业论文.docxVIP

PAGE PAGE IV The roles of TESTIN gene in endometrial cancer and mechanism Abstract The roles of TESTIN gene in endometrial cancer and mechanism Abstract Part I The expression of TESTIN gene in endometrial cancer Objective: To explore the expression of TESTIN gene in endometrial cancer tissues． Methods: Real time PCR and immunohistochemical staining assay were used to determine the mRNA and protein 1evel of TESTIN in the tumor tissues．The relationship between TESTIN expression and clinico-pathological characteristics was analyzed. Methylation-specific PCR was performed to test the promoter status of TESTIN. Results: Compared to the non-cancerous tissues ， more than 75 ％ (52/68) of endometrial cancer tissues presented loss of TESTIN protein (p0.01)，furthermore，this reduction was much more significant in the subgroup of endometrioid adenocarcinoma，but it was rarely observed in the subgroup of serous papillary adenoearcinoma (χ 2=13.34， p=0.001)．As the results of MSP shown, the promoter of TESTIN was hypermethylated in 44.1% (30/68) of endometrial cancer cases, while only 3.6% (1/28) of the non-cancerous tissues showed the hypermethylated TESTIN (p0.001). Moreover, it was found that hypermethylation of TESTIN was significantly correlated to deep myometrial invasion (p=0.007) and lymphatic node metastasis (p=0.03). The downregulation of TESTIN in endometrial cancer was correlated to its hypermethylated promoter (p=0.021), which could be restored by the treatment of 5-aza-dC. Conclusions: TESTIN was significantly downregulated by methylation in endometrial cancer. The loss of TESTIN was mainly caused by the promoter hypermethylation. Keywords: TESTIN gene，tumor suppressor gene，endometrial carcinoma，promoter hypermethylation Abstract The roles of TESTIN gene in endometrial cancer and mechanism PartⅡ The functions of TESTIN in endometrial cancer development and its mechanisms Objective: To explore the mechanisms of TESTIN in the invasion of endometrial cancer.