TERC基因表达情况与HPV感染在宫颈上皮内瘤变的分析-肿瘤学专业论文.docxVIP

摘 要 TERC 基因表达情况与 HPV 感染在宫颈上皮内 瘤变的研究分析 研究生：李晓荣 导师：刘开江 副教授 摘 要 目的：探讨不同级别的宫颈上皮内瘤变中端粒酶 RNA 组分（TERC）的表达情 况及 HPV 感染情况。方法：应用双色间期荧光原位杂交技术（FISH）检测 20 例正 常人及 30 例宫颈上皮内瘤变患者的 TERC 情况。采用导流杂交基因芯片技术对 30 例宫颈上皮内瘤变患者宫颈细胞学进行 HPVDNA 的检测。结果：（1）用 FISH 法对 20 例正常妇女宫颈细胞学 TERC 基因检测，得到 TERC 基因扩增阳性阈值与卫生部 课题小组提供阈值基本相符，为 6 个异常细胞。（2）在 30 例宫颈上皮内瘤变患者中 TERC 基因扩增阳性率（53.3%），说明各组之间总的 TERC 基因扩增阳性率存在统 计学差异（P0.05）。（3）30 例患者中说明 TERC 基因的扩增（53.3%）与 HPV 感染 的阳性概率（56.7%）不相等，差别有统计学意义（P=0.004），同时发现 TERC 基因 的扩增与 HPV 感染之间存在关联性（r=0.585，P=0.003）。结论：HPV 感染及 TERC 基因扩增与宫颈上皮内瘤变的发生发展密切相关，有望成为临床 CIN 诊断及病情监 测的辅助指标，应用于临床早期宫颈病变的诊断中。 关键词：宫颈上皮内瘤变（CIN）；人乳头瘤状病毒（HPV）；端粒酶 RNA 组分（TERC 基因）；荧光原位杂交技术（FISH） —1— 新疆医科大学医学硕士学位论文 Study on Telomerase RNA Component Expression and HPV Infection in Cervical Intraepithelial Neoplasia Postgraduate: LI Xiao-rong Supervisor: Prof. Liu Kai-jiang Abstract Objective: To explore the expression of telomerase RNA component (TERC) and HPV infection in different levels of cervical intraepithelial neoplasia. Methods: The dual - color interphase fluorescence in situ hybridization (FISH) was used to detect the expression of telomerase RNA component(TERC) in 20 cases of normal persons and 30 cases of patients with cervical intraepithelial neoplasia. 30 cases of cytological samples with cervical intraepithelial neoplasia were determined for HPVDNA by flow-through hybridization and gene chip. Results : (1) The threshold of the positive amplification of TERC gene from detecting 20 cases of cytological samples of normal cervix by FISH was in line with the basic threshold from the Ministry of Health team. It was six abnormal cells. (2) The positive rate of TERC gene amplification (53.3%) in 30 cases of cervical intraepithelial neoplasia patients ， indicating that the overall rate of TERC gene amplification among each group has significant difference (p 0.05). (3) The positive rate of TERC gene amplification (53.3%) and the rate of HPV infection (56.7%) are not equal .The difference has statist