Th17细胞调节性T细胞偏倚在肝包虫病所致免疫逃避中意义-外科学专业论文.docxVIP

—4— —4— 新疆医科大学医学硕士学位论文 significance found between CE and RCE groups （t=0.56，P＞0.05）. Th17/Treg （IL-17/IL-10）ratio significantly decreased in CE（3.58 ± 1.52）and RCE（3.41 ± 1.68） than in HC group（5.74 + 2.63）（F=13.76，P＜0.01），whereas no statistical significance found between CE and RCE（t=0.234，P＞0.05）. The serum concentrations of IL-17 were positively correlated to IL-23（r=0.664，P＜0.01）.meanwhile，IL-10 concentrations were positively correlated with TGF-β1 concentration（r=0.344，P＜0.01）. Compared with HC groups（0.111 + 0.603），the expression of ROR-γτ （ROR-γτ copies / GAPDH copies） in CE （0.067 + 0.047）and RCE （0.059 + 0.056）were decreased （F=7.812，P＜0.05）. There were no statistical difference between CE and RCE groups （t=0.56，P＞0.05）. Oppositely，the expression of FoxP3 （FoxP3 copies / GAPDH copies）was increased in CE （0.007 + 0.005）and RCE （0.008 + 0.010）groups than that in HC group（0.006 + 0.005），but had no statistical difference（F=0.226，P＞0.05）. Compared with HC group （24.74 + 19.24），the ratio of ROR-γτ and FoxP3 was obviously decreased in CE（12.64 + 7.59）and RCE（9.69 + 6.10）groups （F=7.179，P＜0.01），but there was no difference between CE and RCE groups （t=0.48，P＞0.05）. Conclusions：Serum concentrations of IL-17 and IL-23 （Th17 cells related cytokines）and the expression of ROR-γτ （Th17cell specific transcription factor）in PBMCs were significantly lower in CE groups than that were in HC group；However，serum concentrations of IL-10 and TGF-β1 （Treg related cytokines）and the expression of FoxP3（Treg cells specific transcription factor） in PBMCs were highly elevated in CE than that were in HC groups. Treg overwhelmed Th17/Treg imbalance were observed in in patients with cystic echinococcosis. The Th17/Treg imbalance may one of the possible mechanisim that echinococcus granulosus used to actively modulate and suppress host immune system so that evade host’s immune attacks for continuous survival. Key words：Hepatic cystic echinococcosis；T helper cell -17；Regul