SOX7基因在口腔鳞癌中的异常表达及甲基化研究-口腔颌面外科专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要····································································································1 英文摘要····································································································3 研究论文 SOX7 基因在口腔鳞癌中的异常表达及甲基化研究 前言····································································································7 材料与方法························································································8 结果··································································································18 附图··································································································20 附表··································································································22 讨论··································································································26 结论··································································································31 参考文献··························································································32 综述 SOX7 基因与肿瘤关系的研究进展·············································35 致谢··········································································································46 个人简历·································································································47 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 SOX7 基因在口腔鳞癌中的异常表达及甲基化研究 摘 要 目的：口腔鳞癌是头颈部常见的恶性肿瘤之一，在我国口腔鳞 癌的发病率在 3.6/10~8.0/10 万。口腔鳞癌对于目前治疗的反应率较 低，使得口腔鳞癌患者的 5 年生存率在 60%左右。较高的死亡率及 较高的第二原发癌发生率使得口腔医生一直在寻找治疗口腔鳞癌的 有效方法。相关研究证实长期吸烟、饮酒及 HPV 病毒感染等都是口 腔鳞癌的发生的高危因素。研究显示表观遗传改变存在于口腔肿瘤 及其他多种肿瘤中。表观遗传改变可导致基因组不稳定和基因表达 的改变，可能与肿瘤的发生密切相关。SOX7 属于 SOX 基因家族 F 亚族，主要参与调节多种发育过程，如心血管形成、内胚层分化及 肌 肉 发 育 等 。 近 年 来 相 关 研 究 报道 SOX7 基 因 甲 基 化 状 态 及 SOX7mRNA 表达异常存在于胃癌、肺癌、前列腺癌等癌组织中， SOX7 基因的异常改变可能参与了癌症的发生及发展。目前尚未发现 SOX7 基因在口腔鳞癌中的相关报道。因此本实验采用甲基化特异性 PCR 及 RT-PCR 分析 SOX7 基因的甲基化状态和 mRNA 的表达情况 与口腔鳞癌发生的关系，初步探索口腔鳞癌的发生机制，并为口腔 鳞癌治疗提供实验数据。 方法： 1 收集河北医科大学第四医院口腔鳞癌组织及正常口腔黏膜组 织标本 48 例，采用甲基化特异性 PCR（Met