VEGF-C和VEGF-D及其受体VEGFR-3表达对大肠癌转移和预后的影响-病理学与病理生理学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 10 英文缩写一览表 缩写 CRC VEGF 英文全称 colorectal cancer vascular endothelial growth factor 中文对照 大肠癌 血管内皮生长因子 VEGF-C vascular endothelial growth factor-C 血管内皮生长因子-C VEGF-D VEGFR-3 VEGFR-2 vascular endothelial growth factor-D vascular endothelial growth factor receptor-3 vascular endothelial growth factor receptor-2 血管内皮生长因子-D 血管内皮生长因子受体-3 血管内皮生长因子受体-2 MLD MVD microlymphatic density microvessel density 微淋巴管密度 微血管密度 IHC SP PBS immunohistochemistry streptamidin-peroxidase phosphate buffer Saline 免疫组织化学 链霉亲和素-过氧化物酶 磷酸盐缓冲液 DAB AEC BCIP diaminnobenzidine 3-amino-9-ethyl carboloe 5-bromo-4-chloro-3-indolyl phosphate 二氨基联苯胺 3-氨基-9-乙基卡巴唑 5-溴-4-氯-3-吲哚磷酸盐 NBT RT-PCR nitro blue tetrazolium reverse transcription PCR 四唑氮蓝 逆转录聚合酶链式反应 mRNA bp cDNA messenger RNA base pair complementary DNA 信使RNA 碱基对 互补 DNA DEPC EDTA diethypyrocabonate ethylene dianinetraccetic acid 焦磷酸二乙酯 乙二胺四乙酸 OD IOD RIOD optical density intergral optical density relative IOD 光密度 积分光密度 相对积分光密度 KD RTK kilo dultun receptor tyrosine kinases 千道尔顿 酪氨酸激酶受体 VHD VEGF homology domain 血管内皮生长因子同源区 The expression of VEGF-C and VEGF-D and their receptor VEGFR-3 in colorectal cancer and its influence to metastasis and prognosis Abstract Vascular endothelial growth factor-CVEGF-Cand VEGF-D are two new members of the vascular endothelial growth factorVEGFfamily of cytokines. It is reported that VEGF-C and -D have lymphangiogenic actions in cancers and can promote lymph node metastasis. Lymphangiogenesis, the development of new lymph vessels, is a relatively new area of clinical investigation. Increased interest in this field has been heightened by the discovery of the two new VEGF family members, which possess lymphangiogenic roles. The role of VEGF-C and VEGF-D in colorectal cancersCRCs remains unclear, with conflicting reports in the published literature. In our study, the protein expression of VEGF-C, VEGF-D, VEGFR-3, podoplanin, CD34 and Ki-67 in CRCs were detected by im