水稻OsDHHC13基因参与氧化胁迫响应初步研究.docVIP

水稻OsDHHC13基因参与氧化胁迫响应初步研究 　　摘 要：DHHC型锌指蛋白参与细胞内蛋白质的棕榈酰化修饰，对细胞和个体的生长发育起着重要的调控作用.OsDHHC13是典型的DHHC型锌指蛋白基因.生物信息学分析发现，OsDHHC13的启动子区域含有胁迫及光响应元件，而且其编码蛋白是具有4个跨膜结构域的典型膜蛋白.对OsDHHC13过表达株系进行H2O2的氧化胁迫处理发现，过表达株系幼苗的根显著长于野生型，说明OsDHHC13能解除H2O2对根生长的抑制，提高了抗氧化胁迫的能力.随后H2O2含量测定发现，OsDHHC13过表达株系幼苗根部的H2O2含量显著低于野生型.进一步的qRTPCR分析发现，与清除H2O2相关的酶基因的转录水平在过表达株系中显著升高，说明OsDHHC13能促进清除H2O2的相关酶基因的表达，调节内源性H2O2的动态平衡.总之，本研究结果初步证明锌指蛋白基因OsDHHC13正调控水稻的抗氧化胁迫能力. 　　关键词： OsDHHC13基因；H2O2含量；根长；锌指蛋白 　　中图分类号：Q943 文献标识码：A 　　Abstract：DHHCtype zinc finger proteins are involved in the palmitoylated modification of intracellular proteins and play important roles in the growth and development of cells and individuals. OsDHHC13 is a typical DHHCtype zinc finger gene. Bioinformatics analysis showed that the OsDHHC13 promoter region contains some stress and light response elements， and its encoded protein is a typical membrane protein with four transmembrane domains. When the OsDHHC13 overexpressing lines were suffered from oxidative stress with H2O2， the roots of overexpressing seedlings were significantly longer than those of wildtype， indicating that OsDHHC13 can remove the inhibition of root growth by H2O2 and improve the ability of resistance to oxidative stress. Subsequently， analysis of H2O2 contents found that the H2O2 contents in the roots of overexpressing seedlings were markedly lower than that in wild type. qRTPCR analysis further revealed that the transcriptional levels of enzyme genes related to H2O2 elimination increased significantly， suggesting that OsDHHC13 can promote the expression of enzyme genes related to H2O2 elimination and regulate the homeostasis of endogenous H2O2 in plants. In a whole， present study preliminarily proved that the zinc finger protein gene OsDHHC13 positively regulates the ability of resistance to oxidative stress. 　　Key words：OsDHHC13 gene；H2O2 content；root length；zinc finger protein 　　DHHC型锌指蛋白是一类富含DHHC （AspHisHisCys） 型锌指结构域的蛋白质.DHHC型锌指结构域是一种富含半胱氨酸高度保守的锌指结构域，是一种常见的DNA结合结构单元.DH