“黄海一号”中国对虾抗白斑病SRAP分子标记微生物学专业论文.docxVIP

Max Indent With EST of immunoglobulin of Ovis Aries and 87% Max Indent with EST of Aldehyde Dehydrogenase of Ictalurus punctatus. We can deduce that they have some relation with anti-WSSV. In summary, the results of this experiment give some theoretical basis for the future prevention and seed breeding of Fenneropenaeus Chinensis. Key Words: Fenneropenaeus Chinensis, White Spot Syndrome Virus, Sequence Related Amplified Polymorphism, seed selection VI 缩略词表 WSSV White Spot Syndrome Virus(白斑综合症病毒) TSV Taura Syndrome Virus（桃拉综合症病毒） IHHNV Infectious Hypodermal and Hematopoietic Virus（传染性皮下及造血 器官坏死病） SRAP Sequence-Related Amplified Polymorphism(相关序列扩增多态性) bp base pair(碱基对) ORF Open Reading Frame(开放阅读框) TEM Transmission Electron Microscopy(透射电镜) PCR Polymerase Chain Reaction(聚合酶链式反应) DIG Digoxigenin(地高辛) ELISA enzyme linked immunosorbent assay(酶联免疫吸附法反法) SPF special pathogen free(无特定病原体) AFLP Amplified Fragment Length Polymorphism(扩增片段长度多态性) CAPS Cleavved Amplified Polymorphic Sequence(酶切扩增片段多态性序 列) TRAP Target Region Amplified Polymorphism(靶位区域扩增多态性) SNP Single Nucleotide Polymorphism(单核苷酸多态性) EST Expressed Sequence Tag(表达序列标签) QTL quantitative trait locus(数量性状定位) Amp ampicillin(氨苄青霉素) Bis Bis-Acrilamide(甲叉双丙烯酰胺) Brph Blue Bromphenol Blue(溴酚蓝) CTAB Hexadecyl Trimethyl Ammonium Bromide(十六烷基三甲基溴化铵) DNA deoxyribonucleic acid(脱氧核糖核酸) dNTP deoxynucleoside triphosphate(脱氧核苷三磷酸) RNA ribonucleic acid (核糖核酸) EB ethidium bromide(溴化乙锭) EDTA ethylene diamine tetraacetic acid(乙二胺四乙酸) IPTG isoporopylthio-β-D-thiogalactoside(异丙基-β-D-硫代半乳糖苷) LB Luria-Bertani medium(Luria-Bertani 培养基) OD600 optical density at 600nm(600nm 处光密度) SDS sodium dodecyl sulfate(十二烷基硫酸钠) PAGE polyacrylamide gel electrophoresis(聚丙烯酰胺凝胶电泳) TAE Tris/acetate electrophoresis buffer(Tris/乙酸电泳缓冲液) TE Tris/EDTA buffer(Tris/EDTA 缓冲液) TEMED N,N,N,N-tetramethyl ethylene diamine(N,N,N,N-四甲基乙二铵) Tm melting temperature(解链温度) Tris trihydroxymethyl aminomethane［三(羟甲基)氨基甲烷］ X-gal 5-bromo-4-chloro-3-inclolyl-D-galactoside(5 溴-4 氟-