不同油荼品种果皮的化学成分与抗炭疽病之间关系的研究-森林保护专业论文.docxVIP

PAGE PAGE IV 等为主；安州小青阴离子化合物有 18 种，有少许的黄酮类和酚类物质，如莽草酸、 绿原酸等为主；阳离子有 28 种化合物，以生物碱类和氨基酸等为主。 关键词：油茶炭疽病，单宁，花青素，可溶性糖，pH 和缓冲容量，酶活，AFLP，遗 传多样性，HPLC-DAD-MS Abstract Camellia oleifera Abel. is an important edible oil tree species from Southern China. Anthracnose, caused by Colletotrichum gloeosporioides (Penz.), is responsible for more than 50% of C. oleifera production loss, and C. oleifera varieties differ in their resistance to anthracnose. 1. The aim of this study was to Assess resistance mechanisms by monitoring physiological and biochemical parameters of differentially resistant cultivars during the development of C. oleifera; 2. Genetic relationship and diversity among different disease-resistant Camellia oleifera varieties were analyzed to further distinguish between varieties at the molecular level; 3. The key bioactive materials which were relavant with resistance were deterimined and studied by HPLC-DAD-MS. The specific research was as follows: C. oleifera fruit coats were analyzed between May and September for tannins, anthocyanins. Tannin contents generally declined at the developmental stage (highly resistant 0.50-0.32, medium resistant 0.46-0.24, highly susceptible 0.48-0.45), whereas no significant difference was found in tannin levels between differentially disease-resistant cultivars (P 0.05). The growth rate of highly resistant varieties was 12.1% higher than of susceptible ones in May. On the contrary, the anthocyanin contents of fruit coats in highly resistant, medium resistant and highly susceptible varieties grew by 77.3%, 69.4% and 51.5%, respectively, and the synthesis rate of anthocyanins changed mildly after August. The anthocyanin contents differed significantly among different cultivars (P 0.01). There was no significant difference in pH levels between the disease-resistant varieties and the susceptible ones at the developmental stage (P 0.05). In July, fruit coats in susceptible varieties reached a pH value of 5, while resistant varietie