巢式RTPCR检测抗癌中药对结肠癌患者外周血CEA mRNA表达作用.docVIP

巢式RTPCR检测抗癌中药对结肠癌患者外周血CEA mRNA表达作用 　　[摘要] 目的：分析外周血中癌胚抗原信使核糖核酸(CEA mRNA)在诊断结肠癌血行转移中的应用价值，评价抗癌中药“卡宁”对结肠癌的疗效。方法：选取结肠癌患者40例作为研究对象，随机分为对照组与实验组，每组20例，应用巢式-逆转录-聚合酶链反应技术(巢式RT-PCR)检测患者服用“卡宁”前后血CEA mRNA的含量。结果：结肠癌患者治疗前，实验组CEA和CEA ｍRNA阳性率分别为32.5％、60.0％；对照组分别为37.5%、55.0％。治疗后，对照组患者CEA和CEA mRNA的阳性率分别为30%、45%；实验组分别为29.6%、41.2%(P＞0.05), 实验组治疗前后CEA与CEA ｍRNA含量有显著性差异(P0.05)。结论：CEA mRNA是一个实用价值较高的诊断消化系统肿瘤血行转移的指标,抗癌中药“卡宁”可有效治疗结肠癌。 　　[关键词] 癌胚抗原；mRNA；结肠癌；转移；中药 　　[中图分类号] R735.3+5[文献标识码]A [文章编号]1673-7210（2008）10（b）-005-03 　　 　　Nested RT-PCR detecting CEA mRNA expression in peripheral blood of colon cancer patients treated by anti-cancer chinese herb 　　CHANG Qing 1, ZHANG Jie-xia 2, ZHENG Li-xia 2, MAO Xiao-ling 2 　　(1.Guangzhou Medical College, Guangzhou 510120, China; 2.The First Affiliated Hospital Guangzhou Medical College of Guangzhou 510120, China) 　　[Abstract] Objective: To analyze the application value of peripheral blood carcino-embryonic antigen messenger ribonucleic acid(CEA mRNA) in the diagnosis of the hematogenous metastasis of colon cancer, and evaluate the therapeutic effect of the anti-cancer traditional chinese herb “Caning” on colon cancer. Methods: 40 patients with colon cancer were selected as the study objects, and then randomly divided into two groups, control group and experimental group, with 20 patients in each group; then nested RT-PCR was used to determine the CEA mRNA in peripheral blood before and after the administration of “Caning”. Results: Before the therapy with “Caning”, the positive rates of CEA and CEA mRNA in experimental group were 32.5% and 60.0% respectively, and that in control group were 37.5% and 55％ respectively. After the therapy with“Caning”, the positive rates of CEA and CEA mRNA in experimental group were 30% and 45% respectively, and that in control group were 29.6% and 41.2%(P＞0.05). A significant difference of the contents of CEA and CEA mRNA before and after the treatment was shown in the experimental group(P0.05). Conclusion: CEA