不同配伍比例交泰丸对2型糖尿病大鼠的治疗作用及分子机制分析-中西医结合临床专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE 10 PAGE 10 GLUT4 蛋白表达，上调 InsRβ、IRS-1 之酪氨酸磷酸化水平，下调 IRS-1 丝氨酸磷酸 化水平最为明显（P0.05）。 结论 交泰丸不同配伍配伍可有效治疗大鼠 T2DM，其作用机制可能与上调骨骼肌组织 InsRβ亚单位、IRS-1 之酪氨酸磷酸化水平，增加 IRS-1、InsRβ亚单位 PI-3K 之 p85 亚基、GLUT4 蛋白表达，下调 IRS-1 丝氨酸磷酸化水平有关。 关键词 交泰丸；配伍；2型糖尿病；胰岛素信号转导通路 Abstract SectionⅠ Effect of different compatibility proportion of Jiaotai Pills on treating type 2 diabetes mellitus in rats Objective To study the effect of different compatibility proportion of Jiaotai Pills on treating type 2 diabetes mellitus (T2DM) in rats. Methods The model of type 2 diabetes mellitus in rats was established by injection of streptozotocin from tail vein and feeding with high fat and high caloric diet. Diabetic rats were randomly divided into model group, Jiaotai Pills group 1 (Rhizoma Coptidis : cinnamon=2:1), Jiaotai Pills group 2 (Rhizoma Coptidis : cinnamon=4:1), Jiaotai Pills group 3 (Rhizoma Coptidis : cinnamon=10:1) and metformin group. Rats in different treatment groups were given by corresponding therapy from gastric tube. Meanwhile normal animal was set as normal control group. Body weight, oral glucose tolerance test (OGTT), blood lipids level including total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C), plasma levels of free fatty acid (FFA) and adiponectin, plasma liver enzymes activity（ALT、AST、 AKP、γ-GT）and pathological results of liver tissue were determined at the eighth week end after experiment. Results Body weight, fasting plasma glucose (FPG), postpradial plasma glucose at one hour (PG-1h), postpradial blood glucose at two hour (PG-2h), plasma levels of TC, TG, LDL-C, FFA and liver enzymes activity were all increased in rats of model group, compared with those in normal control group. Plasma levels of HDL-C and adiponectin were decreased in model group (P0.01). Fatty degeneration of hepatocytes was apparent in liver tissues in rats of model group. Compared with model group, th