开心散对单胺氧化酶活性影响.docVIP

开心散对单胺氧化酶活性影响 　　[摘要]该研究观察抗抑郁中药古方开心散（KXS）在体内外对单胺氧化酶（MAO）活性的影响，从代谢的角度探索KXS升高单胺类神经递质水平的机制。通过在体外酶反应体系和神经胶质细胞C6中分别观察不同浓度的KXS对MAOA和MAOB活性的影响；在动物水平的研究中，分别观察不同浓度的KXS灌胃给药1周、2周和3周后，对正常大鼠和孤养结合慢性不可预见性中等强度应激（CMS）模型大鼠脑线粒体MAOA和MAOB的活性的影响。结果显示，10 g?L-1的KXS在酶反应体系中能显著降低MAOA和MAOB的活性；在C6细胞中，0625～10 g?L-1的KXS对MAOA的活性没有显著影响，但对MAOB的活性有较明显的抑制作用，且呈浓度依赖关系；KXS作用1，2，3周对正常大鼠脑内MAOA和MAOB均没有显著地影响；338 mg?kg-1剂量给药2周和3周后，CMS大鼠脑内MAOA活性较模型组均有所降低（P005），而KXS作用1，2，3周对MAOB没有显著地影响。结果表明开心散在体外对MAOA和MAOB的活性均有一定的影响，在体内对正常大鼠脑内MAOA和MAOB的活性没有影响，对CMS大鼠脑内MAOA有一定抑制作用。 　　[关键词]开心散；单胺氧化酶；代谢；慢性应激 　　[Abstract]To observe the effect of antidepressant medicine prescription， Kaixinsan （KXS） on monoamine oxidase （MAO） activity， and explore the mechanism of KXS in elevating the levels of monoamine neurotransmitter from the perspective of metabolism， in vitro enzyme reaction system and C6 neuroglial cells， the effect of KXS at different concentrations on MAOA and MAOB activity was observed In animal studies， the effect of KXS at different concentrations on MAOA and MAOB activities of brain mitochondrialin normal rats and solitary chronic unpredictable moderate stress （CMS） model rats after intragastric administration for 1， 2， 3 weeks Results showed that 10 g?L-1 KXS could significantly reduce the activity of MAOA and MAOB in enzyme reaction system； and in C6 cells， KXS within 062510 g?L-1 concentration range had no significant effect on the activity of MAOA， but had obvious inhibitory effect on the activity of MAOB in a dose dependent manner KXS had no significant effect on the activity of MAOA and MAOB in brains of normal rats after action for 1， 2， 3 weeks After 2 and 3 weeks treatment with 338 mg?kg-1 dose KXS， MAOA activity in the brain of CMS rats was decreased as compared with the model group （P005）， while KXS had no significant effect on MAOB activity after 1， 2， 3 weeks of treatment The results indicated that KXS had certain effect on in vitro MAOA and MAOB activity， had no effect on brain MAOA and MAOB activity in