异莲心碱对D半乳糖致衰老小鼠抗氧化作用研究.docVIP

异莲心碱对D半乳糖致衰老小鼠抗氧化作用研究 　　[摘要] 目的：探讨异莲心碱对D-半乳糖致衰老小鼠抗氧化的作用。方法：选取昆明种成年小鼠50只，随机将其分为空白组、模型组、异莲心碱低剂量组（10 mg/kg）、高剂量组（20 mg/kg）及维生素C（Vitamin C）对照组，测定小鼠体重和肝脏指数，以小鼠血清和肝脏组织中的 超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性及 MDA 含量为指标，考察异莲心碱在体内的抗氧化性能。结果：与空白组比较，模型组小鼠体重和肝脏指数明显下降，为32.87 g和3.87 mg/g；血清和肝脏组织 SOD和GSH-Px 活性明显降低，其值分别为247 U/ml、408 U/ml和358、319 U/mgpro；MDA含量为20.62 nmol/ml和13.01 nmol/mgpro，明显增加（P＜0.01）；与模型组比较，异莲心碱20 mg/kg可明显拮抗小鼠体重和肝脏指数的降低、增强血清和肝脏组织 SOD和GSH-Px 活性、减少 MDA含量（P＜0.01），其值分别为36.29 g，4.53 mg/g，305、459 U/ml，438、355 U/mgpro和16.47 nmol/ml。异莲心碱低剂量组小鼠肝脏组织GSH-Px 活性为333 U/mgpro，与模型组比较差异无统计学意义。结论：异莲心碱能显著提高D-半乳糖致衰老小鼠的抗氧化能力。 　　[关键词] 异莲心碱；D-半乳糖；小鼠；抗氧化作用 　　[中图分类号] R965[文献标识码]Ａ[文章编号]1673-7210（2011）07（b）-005-03 　　 　　Study on antioxidation of Isoliensinine in D-Galactose-induced aged mice 　　LIU Siling, HAO Yarong, QIN Jian, YU Li 　　Department of Geriatrics, Renmin Hospital of Wuhan University, Hubei Province,Wuhan 430060, China 　　[Abstract] Objective: To study the antioxidative effect of isoliensinine in D-Galactose-induced aged mice. Methods: The fifty mouses were randomly divided into five groups involved in black group, model group, low-dose isoliensinine group (10 mg/kg), high-dose isoliensinine group (20 mg/kg) and the group treated with Vitamin C. The body weight and the liver index were determined and the activities of super-oxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the contents of malonic dialdehyde (MDA) in serum and liver tissue were assayed respectively. Results: Compared with black group, for aging model mice, the body weight and the liver index declined, the data were 32.87 g and 3.87 mg/g, the activities of SOD and GSH-Px in surum and liver tissue also declined, the data were 247, 408 U/ml and 358, 319 U/mgpro, and the contents of MDA raised, the data were 20.62 nmol/ml and 13.01 nmol/mgpro (P＜0.01). In comparison with the model group, the loss of body weight and the liver index were markedly counteracted, the activities of SOD and GSH-Px in surum and l