壮族pcos患者ppar-γ基因外显子6表达的分析-analysis of ppar - γ gene exon 6 expression in zhuang pcos patients.docxVIP

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壮族pcos患者ppar-γ基因外显子6表达的分析-analysis of ppar - γ gene exon 6 expression in zhuang pcos patients.docx

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壮族pcos患者ppar-γ基因外显子6表达的分析-analysis of ppar - γ gene exon 6 expression in zhuang pcos patients

Study of the correlation between the polymorphism of Exon 6 of PPAR-γ Gene and PCOS ABSTRACT Objective: To investigate the expression of PPAR-γ gene at exon 6 of the Zhuang population with PCOS, then there will be a comparison of the expression between Zhuang and Han PCOS patients, to explore it’s ethnic difference. And to discuss the genotype frequencies of PPAR-γ gene at exon 6 of PCOS patients in each syndrome type. Methods: The specimens from 32 PCOS patients in Zhuang population, and 30 PCOS patients in Han population (PCOS group) were collected in this study, the samples from 30 female patients without PCOS (control group) in Han population and Zhuang population were also contained respectively. Total DNA extraction, PCR, electrophoresis, and DNA sequencing were both used to amplify the SNP of the exon 6 in PPAR γ gene. Further analysis between partial clinical characteristics of PCOS patients were recorded. Comparisons were conducted in genotype frequencies of patients with different Chinese medicine’s syndromes. Results: (1)The genotype frequencies of CT/TT and T allele of exon 6 in PPAR-γ gene in the Zhuang cases group were 31%, 23%, and in the control group without PCOS were 10%, 5%, respectively. The genotype frequencies of CT/TT of PCOS was significantly higher than the group without PCOS, the same as the T allele gene frequencies(P0.05). (2)The genotype frequencies of CT/TT and T allele of exon 6 in PPAR-γ gene in the Han cases group were 33%, 23%, and in the group without PCOS were 7%, 6%, respectively. The genotype frequencies of CT/TT and T allele gene was significantly higher in the Han PCOS patients while compared with that in the group without PCOS (control group) (P0.05). (3)The genotype and allele frequencies of exon 6 in PPAR-γ gene did not differ between Zhuang and Han (P0.05). (4)The BMI (27.31?2.85, 27.62?2.24) and FPG (4.92?0.56, 5.15?0.49) of PCOS patients with gene mutation were higher than those that with