扶正化瘀胶囊对心肌梗死大鼠Rock1Rock2表达影响.docVIP

扶正化瘀胶囊对心肌梗死大鼠Rock1Rock2表达影响 　　摘要：目的 观察扶正化瘀胶囊对心肌梗死大鼠RhoA/Rock信号转导通路主要成员Rock1、Rock2表达的影响，探讨其改善心室重构心肌纤维化的可能机制。方法 采用冠状动脉左前降支结扎术制备急性心肌梗死模型，成模大鼠随机分为模型组、扶正化瘀胶囊组和盐酸法舒地尔组，另设假手术组只穿线不结扎作为对照，每组8只。造模后第2日开始给予相应药物干预，连续4周。免疫组化检测心肌组织Rock1、Rock2蛋白表达，实时荧光定量PCR检测Rock1、Rock2 mRNA表达。结果 与假手术组比较，模型组大鼠心肌组织Rock1、Rock2蛋白表达明显升高（P0.05）；与模型组比较，盐酸法舒地尔组和扶正化瘀胶囊组大鼠心肌组织Rock1、Rock2蛋白表达明显降低（P0.05）。与假手术组比较，模型组大鼠心肌组织Rock1 mRNA表达明显升高 （P0.05）；与模型组比较，盐酸法舒地尔组大鼠心肌组织Rock1 mRNA表达明显降低（P0.05），盐酸法舒地尔组和扶正化瘀方组大鼠心肌组织Rock2 mRNA表达明显降低（P0.05）。结论 扶正化瘀胶囊通过降低心肌梗死大鼠梗死边缘区心肌组织Rock1、Rock2的表达，发挥其抗心室重构心肌纤维化的作用。 　　关键词：心肌梗死；心肌纤维化；Rock；扶正化瘀胶囊；大鼠 　　DOI：10.3969/j.issn.1005-5304.2016.09.018 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）09-0074-04 　　Effects of Fuzheng Huayu Capsule on Expressions of Rock1 and Rock2 in Rats with Myocardial Infarction ZHANG Dong-mei， WU Ai-ming， LOU Li-xia， ZHAO Ming-jing， LV Xi-ying， ZHAO Yi-zhou， CHAI Li-min， GAO Yong-hong， SUN Yi-kun， ZHAO Jiu-li （Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing， Dongzhimen Hospital， Beijing University of Chinese Medicine， Beijing 100700， China） 　　Abstract： Objective To observe the effects of Fuzheng Huayu Capsule on the expressions of the main roles， Rock1 and Rock2， in RhoA/Rock signal transduction pathway in rats with myocardial infarction （MI）； To explore the possible mechanism of Fuzheng Huayu Capsule to improve ventricular remodeling in myocardial fibrosis. Methods The MI model was induced by ligation of the left coronary artery. Rats with MI were randomly divided into the model group， Fuzheng Huayu group and Fasudil hydrochloride group. A sham-operation group threading without ligation was setting as a control group， with eight rats in each group. The rats were treated with corresponding medicine for 4 weeks from the second day after modeling. The expression of Rock1， Rock2 and its mRNA were detected by immunohistochemical and real-time PCR method. Results The protein expression of Rock1 and Rock2 in model group were signific