扶正抑瘤汤对肝癌细胞侵袭转移能力影响研究.docVIP

扶正抑瘤汤对肝癌细胞侵袭转移能力影响研究 　　[摘要] 目的 探讨中药扶正抑瘤汤对肝癌细胞侵袭转移能力的影响。 方法 扶正抑瘤汤复合方剂经提取和提纯后处理肝癌细胞，采用MTT法检测肝癌细胞的增殖能力，划痕实验检测肝癌细胞的迁移能力，Transwell实验检测肝癌细胞的侵袭能力。 结果 MTT结果显示，在肝癌细胞HepG2、MHC97-H中，扶正抑瘤汤400 mg/mL组OD值明显低于对照组，差异有统计学意义（P 0.05）；划痕实验证明在肝癌细胞MHC97-H中，扶正抑瘤汤400 mg/mL组肝癌细胞迁移速度明显低于对照组，差异有统计学意义（P 0.05）；Transwell实验证明，在肝癌细胞MHC97-H中，扶正抑瘤汤400 mg/mL组侵袭的肝癌细胞数量明显低于对照组，差异有统计学意义（P 0.05）。 结论 扶正抑瘤汤能抑制肝癌细胞增殖及侵袭转移能力。 　　[关键词] 肝癌；扶正抑瘤汤；增殖；侵袭转移 　　[中图分类号] R273.372 [文献标识码] A [文章编号] 1673-7210（2018）04（a）-0025-04 　　[Abstract] Objective To investigate the effect of Fuzheng Yiliu Decoction on invasion and metastasis of hepatocellular carcinoma cells （HCC）. Methods Hepatocellular carcinoma cells were treated by Fuzheng Yiliu Decoction. MTT assay was used to detect the proliferation of hepatocellular carcinoma cells. The migration ability of HCC cells was detected by scratch test， while the invasion ability of HCC cells was detected by Transwell assay. Results MTT results showed that in HCC lines HepG2， MHC97-H， the proliferation ability of 400 mg/mL Fuzheng Yiliu Decoction group was significantly lower than the control group （P 0.05）. Scratch test demonstrated that in liver cancer cell MHC97-H， the migration rate of liver cancer cells in Fuzheng Yiliu Decoction 400 mg/mL group was significantly lower than that in the control group （P 0.05）. Transwell experiment proved that in liver cancer cell MHC97-H， the number of invading hepatoma cells in the 400 mg/mL Fuzheng Yiliu Decoction group was significantly lower than that in the control group （P 0.05）. Conclusion Fuzheng Yiliu Decoction can inhibit the proliferation， invasion and metastasis of HCC. 　　[Key words] Hepatocellular carcinoma； Fuzheng Yiliu Decoction； Proliferation； Invasion and metastasis 　　肝?胞肝癌（hepatocellular carcinoma，HCC）由于起病隐匿，60%～80%的患者发现时均处于疾病晚期[1]，大量HCC患者因肿瘤转移而丧失手术机会，尽管近年来肝癌的手术切除率已明显提高，然而HCC手术切除5年远处转移率高达80%[2]，局部热消融治疗3年远处转移率高达61%[3-4]，肝移植术后5年远处转移率高达65%[5]。有关HCC的侵袭转移因素及机制的研究是当前的热点。既往研究提示HCC侵袭转移可能与肿瘤血管高密度表达[6]、MAPK通路的激活[7]、细胞基质金属蛋白（M