慢性阻塞性肺疾病患者血清sFas与TNFα水平检测和意义.docVIP

慢性阻塞性肺疾病患者血清sFas与TNFα水平检测和意义 　　【摘要】 目的 探讨血清可溶性Fas蛋白(sFas)及肿瘤坏死因子-α(TNF-α)在慢性阻塞性肺疾病(COPD)发病中的作用及临床意义。 方法 符合COPD诊断标准的急性加重期与稳定期患者各68例,健康对照组20例,采用酶联免疫吸附试验(ELISA)测定血清sFas与TNF-α的水平。结果 ①COPD急性加重期血清sFas及TNF-α水平与稳定期及对照组比较显著升高(P0.01);②急性加重期重度COPD血清sFas及 TNF-α水平较中度COPD显著升高(P0.05);③COPD急性加重期血清sFas及TNF-α水平与气流受限指标第一秒用力呼气容积(FEV1)呈负相关(r值分别是-0.256、-0.364, P均0.05)。结论 sFas及TNF-α通过抑制细胞凋亡参与COPD病理损伤,sFas及TNF-α可作为判定COPD严重程度的参考指标。 　　【关键词】慢性阻塞性肺疾病;可溶性Fas分子(sFas);肿瘤坏死因子-α(TNF-α);凋亡 　　 　　The detection and Clinical signiflcance of sFas and TNF-α in the serums of patients with chronic obstructive pulmonary disease 　　 　　WANG Di,JIANG Li.Epartment of Respiratory,ShengYang The Fourth Hospital Of People, ShengYang 110031,China 　　 　　【Abstract】 Objective To investigate the function and clinical signiflcance of serum soluble Fas ligand (sFas) and Tumor necrosis factor-a (TNF-α) levels in the patients with COPD.Methods Serum levels of sFas and TNF-α were measured in 68 patients patients during acute episode and after disappearance of symptoms and 20 normal control by enzyme-1inked immunosor-bent assay(ELISA). Results ①The serum levels of sFas and TNF-α in the patients with AECOPD showed increasing significantly compared with those in patients with stable episode and in normal group(P0.01);②The serum levels of sFas and TNF-α in the patients with AECOPD showed increasing significantly compared with those in patients with stable episode and in normal group(P0.05);③The serum levels of sFas and TNF-α in the patients with AECOPD were inversely correlated with Forced expiratory volume in one second (FEV1)( r=-0.256、-0.364, P0.05).ConclusionsWe conclude that increased serum sFas and TNF-α may be associated with progression of COPD though inhibit the cell apoptosis. 　　【Key words】 Chronic obstructive pulmonary diseas ;Soluble Fas ligand;Tumor necrosis factor;Apoptosis 　　 　　作者单位:110031沈阳市第四人民医院呼吸内科(王迪); 　　中国医科大学附属一院呼吸内科(姜莉) 　　 　　 　　COPD的发病机制至今尚不清楚[1]。目前越来越多的证据表明由于凋亡调节因子的异常表达引起细胞的凋亡异常在COPD发病中具有重要的促