手术切口局部浸润罗哌卡因复合地佐辛超前镇痛对GM―CSF表达及切口愈合影响.docVIP

手术切口局部浸润罗哌卡因复合地佐辛超前镇痛对GM―CSF表达及切口愈合影响 　　【摘要】 目的：研究地佐辛及地佐辛与罗哌卡因复合使用对粒细胞-巨噬细胞集落刺激因子（GM-CSF）表达及伤口愈合的影响。方法：将健康清洁雄性昆明小鼠100只采用随机数字表法分为生理盐水浸润组（NS组）、0.5%罗哌卡因组（L组）、地佐辛组（D组）及0.5%罗哌卡因复合地佐辛组（Y组），每组25只。L、D、Y组分别于切皮前10 min皮下注射罗哌卡因1 mL/kg，腹腔注射地佐辛5 mg/kg，皮下注射罗哌卡因1 mL/kg复合腹腔注射地佐辛5 mg/kg。L、D、NS组不注射药物部分注射等剂量生理盐水。每组取5只小鼠记录创面愈合时间，20只小鼠于术后72 h记录伤口面积（计算伤口愈合率）并取周围皮肤组织，检测GM-CSF浓度。结果：L、D、Y组切口愈合率、GM-CSF浓度均高于NS组，比较差异均有统计学意义（P0.05）；Y组切口愈合率及GM-CSF浓度均高于L组和D组，比较差异均有统计学意义（P0.05）；Y组切口愈合时间短于D组及L组，比较差异均有统计学意义（P0.05）。结论：0.5%罗哌卡因与地佐辛均能促进GM-CSF表达，进而促进伤口愈合，二者复合使用对GM-CSF表达及伤口愈合作用更佳。 　　【关键词】 罗哌卡因； 地佐辛； GM-CSF； 伤口愈合 　　Effect of Local Infiltration Anesthesia with Ropivacaine and Preemptive Analgesia with Dezocine on the Healing of Incision and the Expression of GM-CSF/QIAO Fen，CHENG Qiao，FAN Zhe-lu.//Medical Innovation of China，2015，12（26）：022-025 　　【Abstract】 Objective：To evaluate the effect of Dezocine and Dezocine combined with Ropivacaine on healing of incision and the expression of GM-CSF.Method：One-hundred healthy and clean male Kunming mice were randomly divided into Normal Sline group（group NS），0.5% Ropivacaine group（group L），Dezocine roup（group D） ，0.5% Ropivacaine and Dezocine group（group Y），each group had 25 mice. 10 minutes before incision ， group L was injected subcutaneously with 0.5% Ropivacaine（1 mL/kg）， group D was injected by intraperitoneal injection with Decoine（5 mL/kg） ， group Y was injected with 0.5% Ropivacaine（1 mL/kg） and Dezocine（5 mL/kg）.The equal volume of Normal Saline was injected into the no injection sections of group L，D，NS.Five mice in each group were chosen for recording the healing time，twenty mice were taken in each group for recording the wound area about 72 hours after operation（measured the rate of wound healing）.The skin tissues around the incision were removed in order to determine the concentration of GM-CSF.Result：The healing time， the rate of wound healing and the concentration of GM-CSF in group L，D，Y were all better than those in group NS，the differences wer