慢性阻塞性肺疾病患者抗菌肽LL―37表达水平检测及意义.docVIP

慢性阻塞性肺疾病患者抗菌肽LL―37表达水平检测及意义 　　[摘要] 目的 检测抗菌肽LL-37慢性阻塞性肺疾病（COPD）患者血清中和诱导痰中的表达水平及其在COPD发病中的作用。 方法 选择27例符合标准的稳定期COPD患者（COPD稳定组），28例符合标准的急性加重期COPD患者（COPD急性加重组）及24例健康对照者（自2014年1～8月），应用ELISA法检测血清中和诱导痰中LL-37的含量，由定量数据分析其与FEV1%和SGRQ 分数的直线相关关系。 结果 与健康对照组相比，COPD稳定组和COPD急性加重组诱导痰中LL-37的含量均明显升高（P0.05）。COPD稳定组和COPD急性加重组诱导痰中LL-37的水平与FEV1%呈负相关（P0.05或P0.01），而与SGRQ 分数正相关（P0.05或P0.01）。 结论 COPD患者诱导痰中LL-37含量升高，且其表达水平与FEV1%和SGRQ 分数有相关性，LL-37可能在COPD的发病中发挥重要作用。 　　[关键词] 慢性阻塞性肺疾病；LL-37；圣乔治呼吸问卷分数；炎症 　　[中图分类号] R562.25 [文献标识码] A [文章编号] 1673-9701（2015）32-0008-03 　　[Abstract] Objective To examine the concentrations of LL-37 in sera and induced sputum from chronic obstructive pulmonary diseases（COPD） patients and to access the role of LL-37 in the pathogenesis of COPD. Methods The LL-37 levels were determined in sera and induced sputum from twenty-seven patients with stable COPD（SCOPD group）， twenty-eight patients with acute exacerbation of COPD（AECOPD group）and twenty-four healthy controls （from Jan to Aug 2014）by ELISA， and then the linear correlations with FEV1% and SGRQ scores were analyzed using quantitative data. Results Compared with healthy control group， the levels of LL-37 in induced sputum were markedly higher in SCOPD group and AECOPD group（P0.05）. Furthermore， the LL-37 levels in induced sputum negatively correlated with FEV1% in both SCOPD group（P0.05）and AECOPD group（P0.01）. On the contrary，the LL-37 levels in induced sputum positively correlated with SGRQ scores in both SCOPD group（P0.05） and AECOPD group（P0.01）. Conclusion The expression levles of LL-37 are increased in induced sputum from COPD patients compared with healthy controls，and correlated with FEV1% and SGRQ scores in COPD patients， suggesting that LL-37 may play an important role in the pathogenesis of COPD. 　　[Key words] Chronic obstructive pulmonary diseases； LL-37； SGRQ scores； Inflammation 　　慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）是一种持续存在的以不完全可逆的气流受限为特征的慢性呼吸道疾病，近年来的研究表明，气道局部浸润的固有免疫细胞如巨噬细胞、树突状细胞和中性粒细