膀胱移行细胞癌组织中TIP30CC3蛋白的表达及其与患者生物学行为的关系.docVIP

膀胱移行细胞癌组织中TIP30CC3蛋白的表达及其与患者生物学行为的关系 　　[摘要] 目的 探讨膀胱移行细胞癌组织中 TIP30/CC3 蛋白的表达及其与患者生物学行为的关系。 方法 选取接受手术治疗的膀胱移行细胞癌（BTCC）存档蜡块 60 例。免疫组化 S-P 法检测 BTCC 标本中 TIP30/CC3 蛋白的表达，并探讨其与患者生物学行为的关系。另取癌旁正常的膀胱组织 10 例（距肿瘤缘 3～5 cm）作对照。 结果 60 例BTCC标本中TIP30/CC3表达阳性19例（31.7%）；10 例癌旁正常的膀胱组织 TIP30/CC3表达阳性9 例（90.0%）。BTCC标本中TIP30/CC3阳性率低于癌旁正常的膀胱组织（χ2=9.84，P0.05），与 UICC 分期和病理分级密切相关（P0.05）。结论 BTCC 组织中 TIP30/CC3 的表达下调或失表达极有可能会促成膀胱癌的发生和发展。BTCC 组织中 TIP30/CC3 的表达与UICC分期和病理分级等生物学行为明显相关，可能参与了膀胱癌发展、转移和浸润的过程，可作为判断其浸润深度、术后复发和预后的评估指标。 　　[关键词] 膀胱移行细胞癌；免疫组化；TIP30/CC3蛋白；生物学行为 　　[中图分类号] R737.14 [文献标识码] A [文章编号] 1673-9701（2016）36-0029-03 　　[Abstract] Objective To investigate the expression of TIP30/CC3 protein in transitional cell carcinoma of bladder and its relationship with the biological behavior in patients. Methods 60 archival paraffin blocks of bladder transitional cell carcinoma（BTCC） treated with surgical treatment were chosen. The expression of TIP30/CC3 protein in BTCC specimens was detected by immunohistochemistry S-P method， and the relationship between TIP30/CC3 protein expression and the biological behavior was explored. Another 10 cases of normal bladder tissue adjacent to the tumor（3-5 cm away from the tumor margin） as the control group. Results TIP30/CC3 expression was positive in 19 cases of 60 BTCC specimens（31.7%）. The positive expression of TIP30/CC3 was found in 9 cases（90.0%） of 10 normal bladder tissues. The positive rate of TIP30/CC3 in BTCC specimens was lower than that in normal bladder tissue（χ2=9.84， P0.05）， but was closely correlated with UICC staging and pathological grade （P0.05）. Conclusion Down-regulation or loss of TIP30/CC3 expression in BTCC tissues may contribute to the development and progression of bladder cancer. The expression of TIP30/CC3 in BTCC is significantly correlated with the biological behavior such as UICC staging and pathological grading， and may be involved in the development， metastasis and infiltration of bladder cancer. The expression of TIP30/CC3 in