脊髓背角瞬时感受器电位香草酸受体1敏感性神经元对不同温度艾灸响应的研究.docVIP

脊髓背角瞬时感受器电位香草酸受体1敏感性神经元对不同温度艾灸响应的研究 　　摘要：目的 观察脊髓背角瞬时感受器电位香草酸受体1（TRPV1）敏感性神经元对不同温度艾灸“足三里”的响应。方法 健康成年雄性大鼠40只，以41、45、49 ℃分别艾灸干预“足三里”，用玻璃微电极探查L4脊髓背角神经元放电并进行细胞外记录；以辣椒素溶剂、辣椒素溶液穴位注射“足三里”，筛选出瞬时感受器电位香草酸受体1（TRPV1）敏感性神经元，观察并比较脊髓背角TRPV1敏感性神经元对3个温度艾灸的响应及其差异。结果 TRPV1敏感性神经元对3组温度均有响应。从响应的神经元性质看，45、49 ℃艾灸主要表现为兴奋，41 ℃艾灸兴奋和抑制的神经元数目相当；从响应的神经元特征看，不同温度对应特定放电频率的神经元：41 ℃为（8.26±2.14）spikes/s、45 ℃为（4.69±2.61）spikes/s、49 ℃为（10.66±5.97）spikes/s；从影响神经元放电频率变化看，41 ℃艾灸干预前后无明显变化，45、49 ℃艾灸干预后放电频率明显加快（P0.01）。结论 脊髓背角TRPV1敏感性神经元对41、45、49 ℃ 3组温度均有响应，响应的特征、性质和强弱与温度有关。 　　关键词：脊髓背角细胞；神经元；艾灸；足三里；温度；大鼠 　　DOI：10.3969/j.issn.1005-5304.2018.03.011 　　中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）03-0048-05 　　Abstract： Objective To observe the response of TRPV1 sensitive neurons in spinal cord under moxibustion of different temperatures in “Zusanli”. Methods Totally 40 healthy male SD rats were chosen and stimulated with moxibustion at “Zusanli” in 41 ℃， 45 ℃ and 49 ℃. Extracellular recording techniques of glass microelectrode was used in the rats’ L4 spinal cord neurons. Capsaicin vehicle and solution was injected into the rats through “Zusanli” to filter the sensitive neuron to TRPV1. The response of sensitive neurons to TRPV1 in spinal cord under moxibustion of three different temperatures was recorded and compared. Results The TRPV1 sensitive neurons responded to all the three temperatures. Viewing from the nature of the responding neurons， the neuron in 45 ℃ and 49 ℃ mainly performed in agitation， while the number of agitated and inhibitory neurons appeared same in 41 ℃. Viewing from the character of the responding neurons， different temperatures correspond to differential discharge frequency： 41 ℃was （8.26±2.14） spikes/s， 45 ℃ was （4.69±2.61） spikes/s， 49 ℃ was （10.66±5.97） spikes/s. Viewing from the change of discharge frequency， the neurons in 41 ℃ was found without obvious change， while the neurons in 45 ℃ and 49 ℃ discharge frequency apparently increased （P0.01）. Conclusion Sensitive neurons in TRPV1 in s