植物雌激素治疗糖尿病肢体缺血性周围神经病变试验研究.docVIP

植物雌激素治疗糖尿病肢体缺血性周围神经病变试验研究 　　[摘要]目的 研究植物雌激素对糖尿病大鼠周围神经病变的影响，探讨植物雌激素治疗糖尿病周围神经病变的可行性及疗效。方法 ①建立大鼠动物模型。②药物干预：植物雌激素治疗组、阴性对照组、糖尿病组分别给予金雀异黄素、神经营养因子、生理盐水肌注。③标本取材与制备：取大鼠胫神经切片光镜和电镜下观察。④图像分析：光镜切片得到有髓纤维的髓鞘和轴突面积，神经内外膜的血管数。电镜切片观察有髓神经纤维和神经营养血管内皮的改变。结果 糖尿病组胫神经的有髓神经纤维和神经营养血管组织形态学出现明显变化、阴性对照组正常，植物雌激素治疗组有轻微变化。结论 植物雌激素治疗糖尿病周围神经病变是可行和有疗效的。 　　[关键词]植物雌激素；糖尿病；周围神经病变 　　[中图分类号]R587.2 [文献标识码]A [文章编号]1674-0742（2015）03（c）-0011-02 　　[Abstract] Objective To study the effect of phytoestrogen on diabetic peripheral neuropathy in rats， and investigate the feasibility and effect of phytoestrogen in the treatment of diabetic peripheral neuropathy. Methods①A rat animal model was established②Drug intervention： Phytoestrogen treatment group， negative control group， diabetes group was treated with intramuscular injection of genistein， neurotrophic factor， physiological saline， respectively.③Sample collection and preparation： Rat tibial nerve sections were collected and observed under light microscope and electron microscope.④Image analysis： Light microscopic sections obtained by myelinated fiber myelin and axon area， the number of inner and outer membrane of vascular nerve. The observation under electron microscope showed that there were changes in myelinated fibers and nerve nutritional vascular endothelium.⑤Statistical analysis： Morphological index of the image was analyzed statistically by variance analysis. Results The myelinated fibers and nerve nutritional vascular histomorphology of tibial nerve in the diabetic group appeared obvious changes， while those of the negative control group were normal， those of phytoestrogen treatment group had a slight change. Conclusion Phytoestrogen is feasible and effective in the treatment of diabetic peripheral neuropathy. 　　[Key words] Phytoestrogen；Diabetic；Peripheral neuropathy 　　该研究探讨植物雌激素治疗糖尿病周围神经病变的可行性及疗效，并从中进一步寻找植物雌激素更好地应用于临床的方向。该科和广东省人民医院整形创伤血管外科合作于2012年5月―2014年3月做了如下研究。现报道如下。 　　1 资料与方法 　　1.1 动物模型的建立 　　（SD）雌性大鼠（200～250 g）禁食一夜后单次腹腔内注射硫脲佐霉素60 mg/kg，1周