水产养殖复合微生物制剂中菌群数量分析.docVIP

水产养殖复合微生物制剂中菌群数量分析 　　摘 要： 对3种市售水产养殖复合微生物制剂进行菌群数量检测，利用选择性计数培养基，采用平板计数法和MPN法，分别对3种微生物制剂中的不同菌群进行计数。结果表明，选择性计数培养基能对复合微生物制剂进行有效的菌群计数，初步确定3种水产养殖复合微生物制剂主要由放线菌、芽孢杆菌、乳酸菌、酵母菌、硝化细菌五大类菌群组成，制剂菌群种类和数量与标注有一定偏差，粉剂中有少量霉菌检出。本研究提供的选择性计数培养基及计数方法能够有效对市售复合微生物制剂产品中不同菌群进行计数，为评估同类产品的安全性和有效性奠定基础。 　　关键词： 水产养殖；复合微生物制剂；微生物菌群；选择性计数 　　中图分类号：S942.3 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2016.05.010 　　Analysis of Microbial Flora Quantity of Compound Microbial Preparations for Aquaculture 　　WEI Chengxiang1，LEI Qi1，ZHANG Furong1，CUI Yi2，GUO Wanping1， LUAN Chunyu1， ZHAO Jing1，QUAN Chunshan1 　　（1. State Ethnic Affairs Commission-Ministry of Education Key Laboratory of Biochemical Engineering， College of Life Science， Dalian Nationalities University， Dalian， Liaoning 116600， China；2. School of Biological Engineering， Dalian Polytechnic University， Dalian， Liaoning 116034， China） 　　Abstract： Microbial flora quantity detection was carried out aiming at three commercial compound microbial preparations for aquaculture. Colony quantity of different flora in three preparations was counted using plate counting method and MPN method with selective colony count media. The results showed that effective colony count for different microbial flora in compound microbial preparations could be achieved using the selective colony count media. Three preparations mainly consist of five categories including actinomyces， bacilli， lactobacilli， yeasts and nitrifying bacteria. However， for categories of flora and colony quantities of different flora there is a certain deviation between detected results and product description in three preparations. A small amount of molds was also detected in the powder preparation. The selective colony count media and counting methods provided by this study could be used for testing commercial compound microbial preparations for aquaculture. This study laid the foundation for evaluating safety and effectiveness of similar products. 　　Key words： aquaculture；compound microbial prepar